LeDuc J W, Antoniades A, Siamopoulos K
Am J Trop Med Hyg. 1986 May;35(3):654-9. doi: 10.4269/ajtmh.1986.35.654.
Epidemiological investigations were conducted following an outbreak of hemorrhagic fever with renal syndrome (HFRS) which occurred in the state of Epirus, northwestern Greece, in July and August 1983. A total of 8 patients were hospitalized during the outbreak; 3 were severely ill and 1 died. A serosurvey made in May 1984 sampled 184 of the approximately 400 residents of the village of Tsepelovo, where 4 patients resided, and found 12 (6.5%) persons, including convalescent sera from 4 patients, with antihantaviral antibody by immunofluorescent antibody (IFA) tests. Small mammal collections found house rats common in the village, but none exhibited anti-hantaviral antibody. Collections in nearby fields and mountains found Apodemus flavicollis rodents common, and 2 (6%) of 33 captured had high IFA anti-hantavirus antibody. Virus isolation attempts from rodent tissues were unsuccessful. Testing of convalescent patients' sera by IFA and plaque reduction neutralization tests indicated that the etiological agent was neither Puumala virus nor Seoul virus, but appears to be a strain of Hantaan virus or perhaps a new virus. The rodent host of this virus may be A. flavicollis, and the distribution of this species corresponds with previously reported cases of severe HFRS described elsewhere in central Europe.
1983年7月和8月，希腊西北部伊庇鲁斯州爆发肾综合征出血热（HFRS）后开展了流行病学调查。疫情期间共有8名患者住院治疗；3人病情严重，1人死亡。1984年5月进行的血清学调查，对4名患者居住的Tsepelovo村约400名居民中的184人进行了采样，通过免疫荧光抗体（IFA）检测发现12人（6.5%）具有抗汉坦病毒抗体，其中包括4名患者的恢复期血清。小型哺乳动物采集发现家鼠在该村庄很常见，但均未表现出抗汉坦病毒抗体。在附近田野和山区的采集发现黄颈姬鼠很常见，33只捕获的黄颈姬鼠中有2只（6%）具有高IFA抗汉坦病毒抗体。从啮齿动物组织进行病毒分离的尝试未成功。通过IFA和蚀斑减少中和试验对恢复期患者血清进行检测表明，病原体既不是普马拉病毒也不是汉城病毒，而似乎是汉坦病毒株或可能是一种新病毒。该病毒的啮齿动物宿主可能是黄颈姬鼠，该物种的分布与中欧其他地方先前报告的严重HFRS病例相符。
