Masson Jean-Francois
Département de chimie, Université de Montréal , C.P. 6128 Succ. Centre-Ville, Montreal, Quebec H3C 3J7, Canada.
Centre for self-assembled chemical structures (CSACS), McGill University , 801 Sherbrooke Street West, Montreal, Quebec H3A 2K6, Canada.
ACS Sens. 2017 Jan 27;2(1):16-30. doi: 10.1021/acssensors.6b00763. Epub 2017 Jan 6.
The design and application of sensors for monitoring biomolecules in clinical samples is a common goal of the sensing research community. Surface plasmon resonance (SPR) and other plasmonic techniques such as localized surface plasmon resonance (LSPR) and imaging SPR are reaching a maturity level sufficient for their application in monitoring biomolecules in clinical samples. In recent years, the first examples for monitoring antibodies, proteins, enzymes, drugs, small molecules, peptides, and nucleic acids in biofluids collected from patients afflicted with a series of medical conditions (Alzheimer's, hepatitis, diabetes, leukemia, and cancers such as prostate and breast cancers, among others) demonstrate the progress of SPR sensing in clinical chemistry. This Perspective reviews the current status of the field, showcasing a series of early successes in the application of SPR for clinical analysis and detailing a series of considerations regarding sensing schemes, exposing issues with analysis in biofluids, and comparing SPR with ELISA, while providing an outlook of the challenges currently associated with plasmonic materials, instrumentation, microfluidics, bioreceptor selection, selection of a clinical market, and validation of a clinical assay for applying SPR sensors to clinical samples. Research opportunities are proposed to further advance the field and transition SPR biosensors from research proof-of-concept stage to actual clinical applications.
用于监测临床样本中生物分子的传感器的设计与应用是传感研究领域的一个共同目标。表面等离子体共振（SPR）以及其他等离子体技术，如局域表面等离子体共振（LSPR）和成像SPR，正达到足以应用于监测临床样本中生物分子的成熟水平。近年来，在从患有一系列疾病（如阿尔茨海默病、肝炎、糖尿病、白血病以及前列腺癌和乳腺癌等癌症）的患者采集的生物流体中监测抗体、蛋白质、酶、药物、小分子、肽和核酸的首批实例，展示了SPR传感在临床化学中的进展。本综述回顾了该领域的现状，展示了SPR在临床分析应用中的一系列早期成功案例，详细阐述了有关传感方案的一系列考量因素，揭示了生物流体分析中存在的问题，并将SPR与酶联免疫吸附测定（ELISA）进行了比较，同时展望了目前与等离子体材料、仪器、微流体、生物受体选择、临床市场选择以及将SPR传感器应用于临床样本的临床检测验证相关的挑战。还提出了研究机会，以进一步推动该领域发展，并使SPR生物传感器从研究概念验证阶段过渡到实际临床应用。
