检测过敏性哮喘患者 PBMCs 中的 IL-9 产生 T 细胞。

Detection of IL-9 producing T cells in the PBMCs of allergic asthmatic patients.

机构信息

Institute of Immunology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China.

The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510600, China.

出版信息

BMC Immunol. 2017 Jul 19;18(1):38. doi: 10.1186/s12865-017-0220-1.

DOI:10.1186/s12865-017-0220-1

PMID:28724400

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5517817/

Abstract

BACKGROUND

Interleukin-9 (IL-9) was reported as an active participant in the pathogenesis of allergic asthma. This study aimed to investigate the major source ofIL-9 and its effect on interferon γ (IFN-γ) and immunoglobulin (Ig) secretion by B cells.

METHODS

We isolated peripheral blood mononuclear cells from children with allergic asthma and healthy children. IL-9, IL-4 and IFN-γ expression were detected by ELISA, ELISpot and Flowcytometry. Expression of transcription factor PU.1 was measured by Western Blot. We evaluated the effect of IL-9 on B cell activation and Ig production.

RESULTS

Results showed that compared with healthy children, levels of IL-9, IL-4 and PU.1 were elevated and levels of IFN-γ were lower in children with allergic asthma. IL-9-expressing CD4 T cells did not co-express IL-4. Exogenous IL-9 inhibited IFN-γ production in a dose-dependent manner. Antigen-specific Th9 cells existed in children with house dust mite allergic asthma. IL-9 up-regulated expression of CD69 and CD25 on B cells and combination of IL-9 and IL-4 enhanced IgE production.

CONCLUSIONS

In conclusion, our results showed that Th9 cells may be the major source of IL-9 in children with allergic asthma. In these patients, IL-9 impairs IFN-γ production and synergistically promotes IL-4-induced IgE secretion.

摘要

背景

白细胞介素-9（IL-9）被报道为过敏性哮喘发病机制中的一个活跃参与者。本研究旨在探讨 IL-9 的主要来源及其对 B 细胞产生干扰素γ（IFN-γ）和免疫球蛋白（Ig）的影响。

方法

我们从过敏性哮喘儿童和健康儿童中分离外周血单个核细胞。通过 ELISA、ELISpot 和流式细胞术检测 IL-9、IL-4 和 IFN-γ的表达。通过 Western Blot 测量转录因子 PU.1 的表达。我们评估了 IL-9 对 B 细胞活化和 Ig 产生的影响。

结果

结果表明，与健康儿童相比，过敏性哮喘儿童的 IL-9、IL-4 和 PU.1 水平升高，IFN-γ 水平降低。表达 IL-9 的 CD4 T 细胞不共表达 IL-4。外源性 IL-9 呈剂量依赖性抑制 IFN-γ 的产生。屋尘螨过敏性哮喘儿童存在抗原特异性 Th9 细胞。IL-9 上调 B 细胞 CD69 和 CD25 的表达，并与 IL-4 协同增强 IgE 的产生。