• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

钙网织蛋白缺失揭示了钙在调节细胞脂质稳态中的关键作用。

Loss of Calreticulin Uncovers a Critical Role for Calcium in Regulating Cellular Lipid Homeostasis.

机构信息

Department of Biochemistry, University of Alberta, Edmonton, Alberta, T6G 2H7, Canada.

Department of Life Sciences, Research Institute for Natural Sciences, BK21 Plus Life Science for BDR Team, Research Institute of Natural Science, Hanyang University, Seoul, 133-791, South Korea.

出版信息

Sci Rep. 2017 Jul 19;7(1):5941. doi: 10.1038/s41598-017-05734-x.

DOI:10.1038/s41598-017-05734-x
PMID:28725049
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5517566/
Abstract

A direct link between Ca and lipid homeostasis has not been definitively demonstrated. In this study, we show that manipulation of ER Ca causes the re-distribution of a portion of the intracellular unesterified cholesterol to a pool that is not available to the SCAP-SREBP complex. The SREBP processing pathway in ER Ca depleted cells remained fully functional and responsive to changes in cellular cholesterol status but differed unexpectedly in basal activity. These findings establish the role of Ca in determining the reference set-point for controlling cellular lipid homeostasis. We propose that ER Ca status is an important determinant of the basal sensitivity of the sterol sensing mechanism inherent to the SREBP processing pathway.

摘要

钙（Ca）和脂质动态平衡之间没有明确的直接联系。在这项研究中，我们表明内质网 Ca 的操纵会导致细胞内未酯化胆固醇的一部分重新分布到不可用于 SREBP 复合物的池子里。内质网 Ca 耗竭细胞中的 SREBP 加工途径仍然保持完全功能，并且对细胞胆固醇状态的变化有反应，但出乎意料的是在基础活性方面有所不同。这些发现确立了 Ca 在确定控制细胞脂质动态平衡的参考设定点方面的作用。我们提出内质网 Ca 状态是固醇感应机制固有 SREBP 加工途径的基础敏感性的重要决定因素。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7966/5517566/699b432d39de/41598_2017_5734_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7966/5517566/7499636f49fa/41598_2017_5734_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7966/5517566/21c223e1edbe/41598_2017_5734_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7966/5517566/79fdab88f4dc/41598_2017_5734_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7966/5517566/36cb93c9d397/41598_2017_5734_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7966/5517566/327af6c70814/41598_2017_5734_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7966/5517566/ae15b2c6d13f/41598_2017_5734_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7966/5517566/c0c571cc8fe4/41598_2017_5734_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7966/5517566/699b432d39de/41598_2017_5734_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7966/5517566/7499636f49fa/41598_2017_5734_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7966/5517566/21c223e1edbe/41598_2017_5734_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7966/5517566/79fdab88f4dc/41598_2017_5734_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7966/5517566/36cb93c9d397/41598_2017_5734_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7966/5517566/327af6c70814/41598_2017_5734_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7966/5517566/ae15b2c6d13f/41598_2017_5734_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7966/5517566/c0c571cc8fe4/41598_2017_5734_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7966/5517566/699b432d39de/41598_2017_5734_Fig8_HTML.jpg

相似文献

1
Loss of Calreticulin Uncovers a Critical Role for Calcium in Regulating Cellular Lipid Homeostasis.钙网织蛋白缺失揭示了钙在调节细胞脂质稳态中的关键作用。
Sci Rep. 2017 Jul 19;7(1):5941. doi: 10.1038/s41598-017-05734-x.
2
Endoplasmic reticulum calcium dictates the distribution of intracellular unesterified cholesterol.内质网钙离子决定细胞内未酯化胆固醇的分布。
Cell Calcium. 2018 Dec;76:116-121. doi: 10.1016/j.ceca.2018.11.002. Epub 2018 Nov 10.
3
COPI-mediated retrieval of SCAP is crucial for regulating lipogenesis under basal and sterol-deficient conditions.衣被蛋白I（COPI）介导的SREBP裂解激活蛋白（SCAP）回收对于在基础和固醇缺乏条件下调节脂肪生成至关重要。
J Cell Sci. 2015 Aug 1;128(15):2805-15. doi: 10.1242/jcs.164137. Epub 2015 Jun 19.
4
Activation of mTOR modulates SREBP-2 to induce foam cell formation through increased retinoblastoma protein phosphorylation.mTOR 的激活调节 SREBP-2，通过增加视网膜母细胞瘤蛋白磷酸化诱导泡沫细胞形成。
Cardiovasc Res. 2013 Dec 1;100(3):450-60. doi: 10.1093/cvr/cvt203. Epub 2013 Sep 25.
5
Calreticulin is an upstream regulator of calcineurin.钙网蛋白是钙调神经磷酸酶的上游调节因子。
Biochem Biophys Res Commun. 2003 Nov 28;311(4):1173-9. doi: 10.1016/j.bbrc.2003.08.040.
6
An ARC/Mediator subunit required for SREBP control of cholesterol and lipid homeostasis.一种SREBP调控胆固醇和脂质稳态所需的ARC/中介体亚基。
Nature. 2006 Aug 10;442(7103):700-4. doi: 10.1038/nature04942. Epub 2006 Jun 21.
7
Involvement of ER-calreticulin-Ca2+ signaling in the regulation of porcine oocyte meiotic maturation and maternal gene expression.内质网钙网蛋白-Ca2+信号在调控猪卵母细胞减数分裂成熟和母源基因表达中的作用。
Mol Reprod Dev. 2010 May;77(5):462-71. doi: 10.1002/mrd.21166.
8
Sterol metabolism and SREBP activation.固醇代谢和 SREBP 激活。
Arch Biochem Biophys. 2010 Sep 15;501(2):177-81. doi: 10.1016/j.abb.2010.06.004. Epub 2010 Jun 10.
9
Different kinetics of cholesterol delivery to components of the cholesterol homeostatic machinery: implications for cholesterol trafficking to the endoplasmic reticulum.胆固醇向胆固醇稳态机制各组分传递的不同动力学：对胆固醇向内质网转运的影响。
Biochim Biophys Acta. 2008 Nov-Dec;1781(11-12):724-30. doi: 10.1016/j.bbalip.2008.08.006. Epub 2008 Sep 17.
10
Calcium binding chaperones of the endoplasmic reticulum.内质网的钙结合伴侣蛋白
Gen Physiol Biophys. 2009;28 Spec No Focus:F96-F103.

引用本文的文献

1
Myeloproliferative Neoplasm-like Mutations of Calreticulin Induce Phenotypes Associated with Calreticulin Dysfunction in .钙网织蛋白样突变诱导. 与钙网织蛋白功能障碍相关的表型
Int J Mol Sci. 2024 Oct 29;25(21):11606. doi: 10.3390/ijms252111606.
2
Identification and Evaluation of qRT-PCR Reference Genes in .. 中qRT-PCR内参基因的鉴定与评估
Insects. 2024 Jul 11;15(7):522. doi: 10.3390/insects15070522.
3
Unraveling the Connection: Cholesterol, Calcium Signaling, and Neurodegeneration.解析关联：胆固醇、钙信号传导与神经退行性变

本文引用的文献

1
Ca(2+) homeostasis and endoplasmic reticulum (ER) stress: An integrated view of calcium signaling.钙离子稳态与内质网应激：钙信号的综合观点
Biochem Biophys Res Commun. 2015 Apr 24;460(1):114-21. doi: 10.1016/j.bbrc.2015.02.004.
2
Three pools of plasma membrane cholesterol and their relation to cholesterol homeostasis.三池质膜胆固醇及其与胆固醇稳态的关系。
Elife. 2014 Jun 11;3:e02882. doi: 10.7554/eLife.02882.
3
Interplay between the oxidoreductase PDIA6 and microRNA-322 controls the response to disrupted endoplasmic reticulum calcium homeostasis.
Neurosci Insights. 2024 May 11;19:26331055241252772. doi: 10.1177/26331055241252772. eCollection 2024.
4
NPC1-dependent alterations in K2.1-Ca1.2 nanodomains drive neuronal death in models of Niemann-Pick Type C disease.NPC1 依赖性 K2.1-Ca1.2 纳米区改变导致尼曼-匹克 C 型疾病模型中的神经元死亡。
Nat Commun. 2023 Jul 28;14(1):4553. doi: 10.1038/s41467-023-39937-w.
5
Transcriptomic Analysis Reveals JAK2/MPL-Independent Effects of Calreticulin Mutations in a Model.转录组分析揭示了钙网蛋白突变在模型中 JAK2/MPL 独立效应。
Cells. 2023 Jan 2;12(1):186. doi: 10.3390/cells12010186.
6
Corrected Serum Ionized Calcium as a Risk Factor Related to Adult Dyslipidemia.校正血清离子钙作为与成人血脂异常相关的危险因素。
Front Cardiovasc Med. 2022 Jul 6;9:916991. doi: 10.3389/fcvm.2022.916991. eCollection 2022.
7
Noncanonical necrosis in 2 different cell types in a Caenorhabditis elegans NAD+ salvage pathway mutant.秀丽隐杆线虫烟酰胺腺嘌呤二核苷酸（NAD+）补救途径突变体中 2 种不同细胞类型的非典型性细胞坏死。
G3 (Bethesda). 2022 Apr 4;12(4). doi: 10.1093/g3journal/jkac033.
8
Caffeine blocks SREBP2-induced hepatic PCSK9 expression to enhance LDLR-mediated cholesterol clearance.咖啡因通过阻断 SREBP2 诱导的肝 PCSK9 表达，增强 LDLR 介导的胆固醇清除。
Nat Commun. 2022 Feb 9;13(1):770. doi: 10.1038/s41467-022-28240-9.
9
Emerging Roles of Calcium Signaling in the Development of Non-Alcoholic Fatty Liver Disease.钙信号在非酒精性脂肪肝病发展中的新作用。
Int J Mol Sci. 2021 Dec 27;23(1):256. doi: 10.3390/ijms23010256.
10
Endoplasmic Reticulum-Based Calcium Dysfunctions in Synucleinopathies.突触核蛋白病中基于内质网的钙功能障碍
Front Neurol. 2021 Oct 20;12:742625. doi: 10.3389/fneur.2021.742625. eCollection 2021.
氧化还原酶PDIA6与微小RNA-322之间的相互作用控制着对内质网钙稳态破坏的反应。
Sci Signal. 2014 Jun 10;7(329):ra54. doi: 10.1126/scisignal.2004983.
4
Sterol regulatory element-binding protein (SREBP) cleavage regulates Golgi-to-endoplasmic reticulum recycling of SREBP cleavage-activating protein (SCAP).固醇调节元件结合蛋白 (SREBP) 裂解调控 SREBP 裂解激活蛋白 (SCAP) 的高尔基体到内质网再循环。
J Biol Chem. 2014 Mar 14;289(11):7547-57. doi: 10.1074/jbc.M113.545699. Epub 2014 Jan 29.
5
Discovery of the cellular and molecular basis of cholesterol control.胆固醇代谢调控的细胞与分子基础研究进展
Proc Natl Acad Sci U S A. 2013 Sep 10;110(37):14833-6. doi: 10.1073/pnas.1312967110. Epub 2013 Aug 23.
6
A conserved SREBP-1/phosphatidylcholine feedback circuit regulates lipogenesis in metazoans.一个保守的 SREBP-1/phosphatidylcholine 反馈回路调节后生动物的脂肪生成。
Cell. 2011 Nov 11;147(4):840-52. doi: 10.1016/j.cell.2011.09.045. Epub 2011 Oct 27.
7
STIM proteins and the endoplasmic reticulum-plasma membrane junctions.STIM 蛋白与内质网-质膜连接点。
Annu Rev Biochem. 2011;80:973-1000. doi: 10.1146/annurev-biochem-061609-165311.
8
Organellar calcium buffers.细胞器钙缓冲剂。
Cold Spring Harb Perspect Biol. 2011 Mar 1;3(3):a004069. doi: 10.1101/cshperspect.a004069.
9
Conserved role of SIRT1 orthologs in fasting-dependent inhibition of the lipid/cholesterol regulator SREBP.SIRT1 同源物在禁食依赖的脂质/胆固醇调节因子 SREBP 抑制中的保守作用。
Genes Dev. 2010 Jul 1;24(13):1403-17. doi: 10.1101/gad.1901210.
10
Mitochondria: the calcium connection.线粒体：钙连接
Biochim Biophys Acta. 2010 Jun-Jul;1797(6-7):607-18. doi: 10.1016/j.bbabio.2010.05.005. Epub 2010 May 12.