Andersson A C, Henningsson S
Acta Physiol Scand. 1981 Dec;113(4):523-32. doi: 10.1111/j.1748-1716.1981.tb06932.x.
The in vivo metabolism of 14C-putrescine injected to rats before, during and after pregnancy was studied. Within 30 min of the administration of the isotope 9-12% of the injected radioactivity was recorded as 14CO2 in the expired air and after 5 h 60% was expired. The radioactivity excreted in the urine during the first day following the 14C-putrescine administration consisted of unmetabolized putrescine, gamma-aminobutyric acid (GABA) and some unidentified compound(s). No radioactive polyamines were detected in the urine. After treatment of pregnant rats with the diamine oxidase inhibitor aminoguanidine the expiration of 14CO2 was almost completely inhibited. In the urine increased amounts of unmetabolized putrescine were excreted while the excretion of GABA and the unidentified compound(s) were decreased. In addition 14C-spermidine appeared in the urine. The in vitro metabolism of putrescine was determined by the incubation of different tissues of pregnant and non-pregnant rats with 14C-putrescine. The 14C-metabolites derived via the diamine oxidase pathway (delta 1-pyrroline, GABA, some unidentified compound(s) and carbon dioxide) varied in magnitude with the tissue investigated. GABA was found to be a main metabolite of putrescine in several tissues of the pregnant rat. The content of putrescine and spermidine was elevated in several tissues as well as the blood on the 19th day of pregnancy in rats treated with aminoguanidine, while the content of spermine was unchanged.
研究了在大鼠怀孕前、怀孕中和怀孕后注射的14C-腐胺的体内代谢情况。在给予同位素后的30分钟内，呼出气体中9%-12%的注入放射性被记录为14CO2，5小时后60%被呼出。在给予14C-腐胺后的第一天，尿液中排出的放射性由未代谢的腐胺、γ-氨基丁酸（GABA）和一些未鉴定的化合物组成。尿液中未检测到放射性多胺。用二胺氧化酶抑制剂氨基胍处理怀孕大鼠后，14CO2的呼出几乎完全受到抑制。尿液中未代谢腐胺的排出量增加，而GABA和未鉴定化合物的排出量减少。此外，尿液中出现了14C-亚精胺。通过将怀孕和未怀孕大鼠的不同组织与14C-腐胺一起孵育来测定腐胺的体外代谢。通过二胺氧化酶途径产生的14C-代谢产物（δ1-吡咯啉、GABA、一些未鉴定的化合物和二氧化碳）的量因所研究的组织而异。在怀孕大鼠的几个组织中，GABA被发现是腐胺的主要代谢产物。在用氨基胍处理的大鼠怀孕第19天时，几个组织以及血液中的腐胺和亚精胺含量升高，而精胺含量不变。
