Instituto de Engenharia de Sistemas e Computadores - Microsistemas e Nanotecnologias (INESC MN) and IN - Institute of Nanoscience and Nanotechnology, Lisbon, Portugal.
Instituto de Engenharia de Sistemas e Computadores - Microsistemas e Nanotecnologias (INESC MN) and IN - Institute of Nanoscience and Nanotechnology, Lisbon, Portugal; IBB - Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Lisbon, Portugal.
Biosens Bioelectron. 2018 Jan 15;99:40-46. doi: 10.1016/j.bios.2017.07.032. Epub 2017 Jul 15.
The field of microfluidics holds great promise for the development of simple and portable lab-on-a-chip systems. The use of capillarity as a means of fluidic manipulation in lab-on-a-chip systems can potentially reduce the complexity of the instrumentation and allow the development of user-friendly devices for point-of-need analyses. In this work, a PDMS microchannel-based, colorimetric, autonomous capillary chip provides a multiplexed and semi-quantitative immunodetection assay. Results are acquired using a standard smartphone camera and analyzed with a simple gray scale quantification procedure. The performance of this device was tested for the simultaneous detection of the mycotoxins ochratoxin A (OTA), aflatoxin B1 (AFB1) and deoxynivalenol (DON) which are strictly regulated food contaminants with severe detrimental effects on human and animal health. The multiplexed assay was performed approximately within 10min and the achieved sensitivities of<40, 0.1-0.2 and<10ng/mL for OTA, AFB1 and DON, respectively, fall within the majority of currently enforced regulatory and/or recommended limits. Furthermore, to assess the potential of the device to analyze real samples, the immunoassay was successfully validated for these 3 mycotoxins in a corn-based feed sample after a simple sample preparation procedure.
微流控技术在简单、便携的芯片实验室系统的发展方面具有广阔的前景。在芯片实验室系统中，利用毛细作用作为流体操控手段，可以降低仪器的复杂性，并开发出适用于即时检测的用户友好型设备。在这项工作中，我们设计了一种基于 PDMS 微通道的比色、自主式毛细芯片，用于实现多重、半定量的免疫检测分析。该设备的检测结果可由标准智能手机摄像头获取，并通过简单的灰度量化程序进行分析。该设备的性能已通过同时检测严格监管的食品污染物——赭曲霉毒素 A（OTA）、黄曲霉毒素 B1（AFB1）和脱氧雪腐镰刀菌烯醇（DON）进行了测试，这些污染物对人类和动物健康具有严重的不利影响。多重检测分析大约可在 10min 内完成，所实现的 OTA、AFB1 和 DON 的灵敏度分别<40、0.1-0.2 和<10ng/mL，均处于目前实施的监管和/或推荐标准范围内。此外，为了评估该设备分析实际样品的潜力，我们在简单的样品制备后，成功地在基于玉米的饲料样品中对这 3 种霉菌毒素进行了免疫检测分析。
