盒外的结构生物学-细胞内。
Structural Biology outside the box-inside the cell.
机构信息
Max Planck Institute of Biochemistry, Department of Molecular Structural Biology, Am Klopferspitz 18, D-82152 Martinsried, Germany.
Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland.
出版信息
Curr Opin Struct Biol. 2017 Oct;46:110-121. doi: 10.1016/j.sbi.2017.06.007. Epub 2017 Jul 21.
Recent developments in cellular cryo-electron tomography, in-cell single-molecule Förster resonance energy transfer-spectroscopy, nuclear magnetic resonance-spectroscopy and electron paramagnetic resonance-spectroscopy delivered unprecedented insights into the inner workings of cells. Here, we review complementary aspects of these methods and provide an outlook toward joint applications in the future.
近年来，细胞冷冻电子断层成像技术、细胞内单分子Förster 共振能量转移光谱学、核磁共振光谱学和电子顺磁共振光谱学的发展为深入了解细胞的内部运作提供了前所未有的视角。在这里，我们回顾了这些方法的互补方面，并展望了未来联合应用的前景。
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