Departamento de Polímeros y Biopolímeros, CINVESTAV Unidad Querétaro, Libramiento Norponiente No. 2000, Fracc. Real de Juriquilla, C.P. 76230, Querétaro, Mexico.
Escuela de Ciencias de la Salud, Universidad del Valle de México (UVM) Campus Juriquilla, Blvd. Juriquilla No. 1000, C.P. 76320, Querétaro, Mexico.
Int J Biol Macromol. 2017 Dec;105(Pt 1):1241-1249. doi: 10.1016/j.ijbiomac.2017.07.159. Epub 2017 Jul 28.
We report the combined antibacterial/tissue regeneration responses to thermal burns promoted by functional chitosan/silver nanocomposites (CS/nAg) with ultralow silver content (0.018wt.%, 7-30nm). Our approach allows one to produce CS/nAg nanocomposites without silver nanoparticles (nAg) agglomeration, with bactericide potency higher than 1wt.% of nAg (ca. 10nm) content and, promoting the healing process in controlled thermal burns. CS/nAg films exhibit high antibacterial activity against S. aureus and P. aeruginosa after 1.5h of incubation, demonstrating the bacterial penetration into hydrated films and their interaction with nAg. Additionally, exceptional healing of induced thermal burns was obtained by increasing myofibroblasts, collagen remodeling, and blood vessel neoformation. These factors are associated with epiderma regeneration after 7days of treatment with no nAg release. Our results corroborate the controlled synthesis of nAg embedded in CS matrix with combined antibacterial/biocompatibility properties aiming to produce functional nanocomposites with potential use in wound dressing and health care applications.
我们报告了功能壳聚糖/银纳米复合材料（CS/nAg）对低热烧伤的联合抗菌/组织再生反应，其银含量超低（0.018wt.%，7-30nm）。我们的方法允许生产无银纳米颗粒（nAg）团聚的 CS/nAg 纳米复合材料，其杀菌效力高于 1wt.%的 nAg（约 10nm）含量，并促进受控热烧伤的愈合过程。CS/nAg 薄膜在孵育 1.5 小时后对金黄色葡萄球菌和铜绿假单胞菌表现出高抗菌活性，表明细菌穿透水合薄膜并与 nAg 相互作用。此外，通过增加肌成纤维细胞、胶原重塑和血管新生，获得了诱导性热烧伤的极好愈合。这些因素与治疗 7 天后表皮再生有关，没有 nAg 释放。我们的结果证实了在 CS 基质中嵌入 nAg 的受控合成，具有联合抗菌/生物相容性特性，旨在生产具有潜在用途的功能性纳米复合材料，用于伤口敷贴和医疗保健应用。
