College of Pharmacy, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, Republic of Korea.
College of Pharmacy, Dankook University, 119 Dandae-ro, Dongnam-gu, Cheonan-si, Chungnam 31116, Republic of Korea.
J Immunol Res. 2017;2017:7052560. doi: 10.1155/2017/7052560. Epub 2017 Jul 2.
The roots of , which belong to the family Euphorbiaceae, have been used as a traditional medicine for the treatment of various diseases such as diabetes, ascites, and leukemia. Recently, it was reported that the methylene chloride fraction of radix (EKC) regulated the differentiation of Th17 cells and alleviated the symptoms of Th17-related inflammatory bowel disease. Imiquimod (IMQ), a TLR7/8 agonist, has been used to induce psoriasis in a mouse model. In this study, we evaluated the effect of EKC in an IMQ-induced psoriasis model. EKC effectively inhibited the production of interleukin-17A and interferon- in vitro. On this basis, EKC was administered to an animal model of psoriasis. Acanthosis and the infiltration of inflammatory cells into the dermis were significantly reduced by EKC. EKC also inhibited the expression of IL-17A, IL-22, IL-23, IL-12, and RAR-related orphan receptor gamma t (RORt) in the spleen, skin-draining lymph nodes, and the skin. Additionally, EKC inhibited the activity of dendritic cells but not that of keratinocytes. In conclusion, EKC ameliorated the symptoms of psoriasis through inhibition of Th17 differentiation and activation of dendritic cells. These effects are expected to be beneficial in the treatment and prevention of psoriasis.
作为大戟科的一种植物， 的根已被用作治疗各种疾病的传统药物，如糖尿病、腹水和白血病。最近有报道称， 根的二氯甲烷馏分（EKC）可调节 Th17 细胞的分化，并缓解与 Th17 相关的炎症性肠病的症状。咪喹莫特（IMQ）是一种 TLR7/8 激动剂，已被用于诱导小鼠模型中的银屑病。在这项研究中，我们评估了 EKC 在 IMQ 诱导的银屑病模型中的作用。EKC 可有效抑制白细胞介素-17A 和干扰素- 的产生。在此基础上，将 EKC 施用于银屑病动物模型。EKC 可显著减少表皮增生和真皮炎症细胞浸润。EKC 还可抑制脾脏、皮肤引流淋巴结和皮肤中 IL-17A、IL-22、IL-23、IL-12 和 RAR 相关孤儿受体γ t（RORt）的表达。此外，EKC 抑制树突状细胞的活性，但不抑制角质形成细胞的活性。总之，EKC 通过抑制 Th17 分化和激活树突状细胞来改善银屑病的症状。这些作用有望有益于银屑病的治疗和预防。
