University of Lodz, Department of Invertebrate Zoology and Hydrobiology, Łódź, Poland.
University of Guelph, Centre for Biodiversity Genomics, Guelph, Ontario, Canada.
Genome. 2021 Mar;64(3):196-206. doi: 10.1139/gen-2019-0226. Epub 2020 Jun 5.
We present the results of the first-ever DNA barcoding study of odonates from the Maltese Islands. In total, 10 morphologically identified species were collected during a two-week long expedition in 2018. Eighty cytochrome oxidase subunit I (COI) barcodes were obtained from the collected specimens. Intra- and interspecific distances ranged from 0.00% to 2.24% and 0.48% to 17.62%, respectively. Successful species identification based on ascribing a single morphological species to a single Barcode Index Number (BIN) was achieved for eight species (80%). In the case of two species, and , BINs were shared with other closely related species. The taxonomic status of is questionable and the phylogenetic relationship between / is not clear. Further studies involving a series of adult specimens collected in a wide spatial range and nuclear markers are necessary to resolve these cases. Therefore, this dataset serves as an initial DNA barcode reference library for Maltese odonates, within a larger project: Aquatic Macroinvertebrates DNA Barcode Library of Malta.
我们呈现了首次对马耳他群岛蜻蜓目昆虫进行 DNA 条形码研究的结果。在 2018 年为期两周的考察中，共采集到 10 种形态学上确定的物种。从采集的标本中获得了 80 个细胞色素氧化酶亚基 I（COI）条形码。种内和种间的距离分别为 0.00%至 2.24%和 0.48%至 17.62%。成功地根据将单一形态物种分配给单个条形码索引编号（BIN）来识别 8 个物种（80%）。在两种情况下， 和 ，BIN 与其他密切相关的物种共享。 的分类地位值得怀疑， / 的系统发育关系不清楚。需要进一步的研究，包括在广泛的空间范围内收集的一系列成虫标本和核标记，以解决这些情况。因此，该数据集作为马耳他蜻蜓目昆虫的初始 DNA 条形码参考文库，是更大项目：马耳他水生大型无脊椎动物 DNA 条形码文库的一部分。
