Department of Food Science and Engineering, School of Chemical Engineering, Northwest University, Xi'an 710069, Shaanxi, China.
Institute of Apiculture Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China.
Food Chem. 2017 Dec 15;237:225-231. doi: 10.1016/j.foodchem.2017.05.065. Epub 2017 May 15.
Hydrogen peroxide (HO) is a major antibacterial activity-associated biomarker in honey. Measurement of endogenous HO in honey is of great value in prediction of the HO-depended antibacterial activity and characterization or selection of honey samples for their use as an antibacterial agent or natural food preservative. Considering current methods for HO determination are either time-consuming or complicated with their high-cost, a study was conducted to modify and validate the spectrophotometry-based ferrous oxidation-xylenol orange (FOX-1) method for micro-determination of HO in honey samples. The result suggested that the proposed FOX-1 method is fast, sensitive, precise and repeatable. The method was successfully applied for the analysis of a total of 35 honey samples from 5 floral origins and 33 geographical origins. The proposed method is low-cost and easy-to-run, and it can be considered by researchers and industry for routine analysis.
过氧化氢（HO）是蜂蜜中主要的与抗菌活性相关的生物标志物。测量蜂蜜中内源性 HO 的含量对于预测 HO 依赖性抗菌活性以及对蜂蜜样品进行特征描述或选择以将其用作抗菌剂或天然食品防腐剂具有重要意义。鉴于目前测定 HO 的方法要么耗时，要么成本高昂且复杂，因此进行了一项研究，旨在对基于分光光度法的亚铁氧化-二甲苯酚橙（FOX-1）法进行修改和验证，以用于微定量测定蜂蜜样品中的 HO。结果表明，所提出的 FOX-1 方法快速、灵敏、精确且可重复。该方法成功应用于分析来自 5 种花源和 33 个地理来源的总共 35 个蜂蜜样品。该方法成本低，易于运行，研究人员和行业可以考虑将其用于常规分析。
