Ramos Bruna Alves, Chiang Jannifer Oliveira, Martins Lívia Carício, Chagas Liliane Leal das, Silva Franko de Arruda E, Ferreira Milene Silveira, Freitas Maria Nazaré Oliveira, Alcantara Bianca Nascimento de, Silva Sandro Patroca da, Miranda Stefânia Araújo, Sepulvreda Barbara Alves, Corrêa Layna Thayssa Guimarães, Negrão Andréa Maria Góes, Vasconcelos Pedro Fernando da Costa, Casseb Alexandre do Rosário
Universidade Federal do Pará, Instituto de Ciências Biológicas, Belém, PA, Brasil.
Instituto Evandro Chagas, Setor de Arbovirologia e Febres Hemorrágicas, Ananindeua, Pará, Brasil.
Mem Inst Oswaldo Cruz. 2017 Aug;112(8):532-536. doi: 10.1590/0074-02760170014.
In this study, we evaluated the role of free-living domestic pigeons (Columba livia) as a reservoir of arboviruses in the city of Belém, state of Pará, Brazil. We investigated the presence of antibodies against the most prevalent arboviruses.
This study was aimed at evaluating some clinical and physical parameters of domestic pigeons, including the presence of antibodies to Amazon-endemic arboviruses.
Eighty-five healthy pigeons were captured in Mangal das Garças Park, in Belém, and were bled. Upon capture, the birds were subjected to a clinical examination in search of alterations that could indicate the presence of arboviruses. Blood samples were converted to serum and tested using the haemagglutination inhibition (HI) technique with a panel of 19 antigens of arboviruses circulating in the Amazon. The confirmation assay for the positive reactions to the viral species tested by HI was a neutralisation test in new-born Swiss albino mice (Mus musculus) [mouse neutralisation test (MNT)].
A total of 10 (11.8%) serum samples tested positive for antiflavivirus antibodies by HI. All the samples positive for the HI test were subjected to MNT for detection of viruses and yielded negative results (logarithmic neutralisation index < 1.7).
The results represent the first serological detection of antiarbovirus antibodies in domestic pigeons as potential hosts of arboviruses in Brazil. The detection of haemagglutination-inhibiting antibodies against genus Flavivirus indicated that there was recent contact between the analysed domestic pigeons and these arboviruses. Further studies are needed to evaluate the role of free-living pigeons in the maintenance cycle and spread of arboviruses in the Amazon.
在本研究中，我们评估了散养家鸽（Columba livia）作为巴西帕拉州贝伦市虫媒病毒宿主的作用。我们调查了针对最常见虫媒病毒的抗体的存在情况。
本研究旨在评估家鸽的一些临床和身体参数，包括针对亚马逊地区特有的虫媒病毒的抗体的存在情况。
在贝伦市的曼加尔达斯加尔萨公园捕获了85只健康鸽子并采集血液。捕获时，对这些鸽子进行临床检查，以寻找可能表明存在虫媒病毒的变化。血液样本转化为血清后，使用血凝抑制（HI）技术，用一组在亚马逊地区传播的19种虫媒病毒抗原进行检测。对通过HI检测的病毒种类呈阳性反应的确认试验是在新生瑞士白化小鼠（Mus musculus）中进行中和试验[小鼠中和试验（MNT）]。
共有10份（11.8%）血清样本通过HI检测呈抗黄病毒抗体阳性。所有HI检测呈阳性的样本都进行了MNT以检测病毒，结果均为阴性（对数中和指数<1.7）。
这些结果代表了在巴西，首次对作为虫媒病毒潜在宿主的家鸽进行抗虫媒病毒抗体的血清学检测。针对黄病毒属的血凝抑制抗体的检测表明，所分析的家鸽与这些虫媒病毒最近有接触。需要进一步研究来评估散养家鸽在亚马逊地区虫媒病毒的维持循环和传播中的作用。
