Department of Microelectronics, Nankai University, Tianjin 300350, China.
Department of Biology, Nankai University, Tianjin 300071 China.
Biosens Bioelectron. 2018 Jan 15;99:251-258. doi: 10.1016/j.bios.2017.07.065. Epub 2017 Jul 27.
In this work, photoelectrochemical (PEC) sensors based on carbon dots (CDs) were developed for ultrasensitive detection of glutathione (GSH) without additional catalysts. In this PEC sensing system, CDs exhibited both photoelectric and catalytic properties. Silver nanoparticles (AgNPs), graphene oxide (GO), and mesoporous silica (MS) were introduced in order to enhance the sensing properties of CDs for GSH. Among the different hybrid nanocomposites, CDs@MS based PEC sensors exhibited the best sensing properties: the sensitivity and limit of detection (LOD) for GSH were found to be 57.6nAμM and 6.2nM (S/N = 3), respectively, in the linear range 0.02-4μM. In addition, the developed PEC sensors showed a high selectivity for GSH even with interferences of other biological thiols and amino acids. The PEC sensor was successfully applied for GSH detection in human serum and probing of myocardial infarction (MI) conditions by estimating the amount of GSH in the myocardial cells of mice, which had been treated with different ischemia/ischemia-reperfusion times. These results indicated that the CDs based hybrid nanocomposites are promising candidates for the development of PEC biosensors with enhanced sensing performances.
在这项工作中，开发了基于碳点 (CDs) 的光电化学 (PEC) 传感器，无需额外的催化剂即可用于谷胱甘肽 (GSH) 的超灵敏检测。在这个 PEC 传感系统中，CDs 表现出光电和催化特性。为了提高 CDs 对 GSH 的传感性能，引入了银纳米粒子 (AgNPs)、氧化石墨烯 (GO) 和介孔硅 (MS)。在不同的杂化纳米复合材料中，基于 CDs@MS 的 PEC 传感器表现出最好的传感性能：在 0.02-4μM 的线性范围内，GSH 的灵敏度和检测限 (LOD) 分别为 57.6nAμM 和 6.2nM（S/N = 3）。此外，开发的 PEC 传感器对 GSH 具有很高的选择性，即使存在其他生物硫醇和氨基酸的干扰也是如此。该 PEC 传感器成功应用于人血清中的 GSH 检测，并通过估计不同缺血/再灌注时间处理的小鼠心肌细胞中的 GSH 量来探测心肌梗死 (MI) 情况。这些结果表明，基于 CDs 的杂化纳米复合材料是开发具有增强传感性能的 PEC 生物传感器的有前途的候选材料。
