El Hadad Amir A, Peón Eduardo, García-Galván Federico R, Barranco Violeta, Parra Juan, Jiménez-Morales Antonia, Galván Juan Carlos
Centro Nacional de Investigaciones Metalúrgicas (CSIC), Madrid 28040, Spain.
Biophysics Branch, Physics Department, Al-Azhar University, Nasr City, Cairo 11884, Egypt.
Materials (Basel). 2017 Jan 24;10(2):94. doi: 10.3390/ma10020094.
The aim of this work was to prepare hydroxyapatite coatings (HAp) by a sol-gel method on Ti6Al4V alloy and to study the bioactivity, biocompatibility and corrosion protection behaviour of these coatings in presence of simulated body fluids (SBFs). Thermogravimetric/Differential Thermal Analyses (TG/DTA) and X-ray Diffraction (XRD) have been applied to obtain information about the phase transformations, mass loss, identification of the phases developed, crystallite size and degree of crystallinity of the obtained HAp powders. Fourier Transformer Infrared Spectroscopy (FTIR) has been utilized for studying the functional groups of the prepared structures. The surface morphology of the resulting HAp coatings was studied by Scanning Electron Microscopy (SEM). The bioactivity was evaluated by soaking the HAp-coatings/Ti6Al4V system in Kokubo's Simulated Body Fluid (SBF) applying Inductively Coupled Plasma (ICP) spectrometry. 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) and Alamar blue cell viability assays were used to study the biocompatibility. Finally, the corrosion behaviour of HAp-coatings/Ti6Al4V system was researched by means of Electrochemical Impedance Spectroscopy (EIS). The obtained results showed that the prepared powders were nanocrystalline HAp with little deviations from that present in the human bone. All the prepared HAp coatings deposited on Ti6Al4V showed well-behaved biocompatibility, good bioactivity and corrosion protection properties.
本工作的目的是通过溶胶-凝胶法在Ti6Al4V合金上制备羟基磷灰石涂层（HAp），并研究这些涂层在模拟体液（SBF）存在下的生物活性、生物相容性和耐腐蚀性能。已应用热重/差热分析（TG/DTA）和X射线衍射（XRD）来获取有关相变、质量损失、所形成相的鉴定、所得HAp粉末的微晶尺寸和结晶度的信息。利用傅里叶变换红外光谱（FTIR）研究制备结构的官能团。通过扫描电子显微镜（SEM）研究所得HAp涂层的表面形貌。通过将HAp涂层/Ti6Al4V系统浸泡在 Kokubo模拟体液（SBF）中并应用电感耦合等离子体（ICP）光谱法来评估生物活性。使用3-[4,5-二甲基噻唑-2-基]-2,5-二苯基溴化四氮唑（MTT）和阿拉玛蓝细胞活力测定法来研究生物相容性。最后，通过电化学阻抗谱（EIS）研究HAp涂层/Ti6Al4V系统的腐蚀行为。所得结果表明，制备的粉末为纳米晶HAp，与人体骨骼中的HAp相差不大。所有沉积在Ti6Al4V上的制备的HAp涂层均表现出良好的生物相容性、良好的生物活性和耐腐蚀性能。
