Illescas-Montes Rebeca, Melguizo-Rodríguez Lucía, Manzano-Moreno Francisco Javier, García-Martínez Olga, Ruiz Concepción, Ramos-Torrecillas Javier
Biomedical Group (Bio277), Department of Nursing, Faculty of Nursing, University of Granada Campus from Melilla, C/Santander, 1, Melilla 58071, Spain.
Instituto de Investigación Biosanitaria ibs.GRANADA, University of Granada, C/Doctor Azpitarte 4, planta, Granada 18012, Spain.
Materials (Basel). 2017 Jul 13;10(7):793. doi: 10.3390/ma10070793.
Fibroblasts are the main cells involved in regeneration during wound healing. The objective was to determine the effect of 940 nm diode laser on cultured human fibroblasts using different irradiation regimens.
The CCD-1064Sk human epithelial fibroblast cell line was treated with a 940 nm diode laser at different energy doses (power: 0.2-1 W and energy density: 1-7 J/cm²) using different transmission modes (continuous or pulsed). The effect on cell growth at 24 and 72 h post-treatment was examined by measuring the proliferative capacity, the impact on the cell cycle, and the effect on cell differentiation.
fibroblast proliferative capacity was increased at 24 and 72 h post-treatment as a function of the energy dose. The greatest increase was observed with a power of 0.2 or 0.5 W and energy density between 1 and 4 J/cm²; no difference was observed between continuous and pulsed modes. There were no significant differences in cell cycle between treated groups and controls. α-actin expression was increased by treatment, indicating enhanced cell differentiation.
The 940 nm diode laser has biostimulating effects on fibroblasts, stimulating proliferative capacity and cell differentiation without altering the cell cycle. Further researches are necessary to explore its potential clinical usefulness in wound healing.
成纤维细胞是伤口愈合过程中参与再生的主要细胞。目的是使用不同的照射方案来确定940nm二极管激光对培养的人成纤维细胞的影响。
使用不同的传输模式（连续或脉冲），以不同的能量剂量（功率：0.2 - 1W，能量密度：1 - 7J/cm²）对CCD - 1064Sk人上皮成纤维细胞系进行940nm二极管激光处理。通过测量增殖能力、对细胞周期的影响以及对细胞分化的影响，来检测处理后24小时和72小时对细胞生长的影响。
处理后24小时和72小时，成纤维细胞的增殖能力随能量剂量增加。在功率为0.2或0.5W且能量密度在1至4J/cm²之间时观察到最大的增加；连续和脉冲模式之间未观察到差异。处理组和对照组之间的细胞周期没有显著差异。处理后α - 肌动蛋白表达增加，表明细胞分化增强。
940nm二极管激光对成纤维细胞具有生物刺激作用，可刺激增殖能力和细胞分化，而不改变细胞周期。有必要进一步研究其在伤口愈合中的潜在临床应用价值。
