Zhou Wen, Ren Biao, Zhou Xuedong, Xu Hockin H K, Weir Michael D, Li Mingyun, Feng Mingye, Li Jiyao, Xu Xin, Cheng Lei
State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610000, China.
Department of Operative Dentistry and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610000, China.
Materials (Basel). 2016 Aug 9;9(8):674. doi: 10.3390/ma9080674.
This study was set to assess the possible benefits of novel cavity disinfectants with 5% dimethylaminododecyl methacrylate (DMADDM); and compare the effectiveness of saliva microbial-aging method with water-aging in measuring the changing of resin-dentin bond strength. Three cavity disinfectants were tested: 0.2% Chlorhexidine (CHX); 5% DMADDM; and 5% DMADDM + 0.2% CHX. Microtensile bond strength (μTBS) test was performed after microbial-aging with saliva microbial or water aging for one month. Hydroxyproline (HYP), the production of collagen degradation, was measured spectrophotometrically. Additionally, the antibacterial effects of each reagent were evaluated. The 5% DMADDM exerted the least percentage of resin-dentin bond strength loss after one month microbial-aging ( < 0.05). There were no statistically significant differences of bond strength decrease after one month water aging among the tested groups ( > 0.05). Microbial-aging method yield more drop of bond strength than water aging in all groups except 5% DMADDM ( < 0.05). Meanwhile, 5% DMADDM had the same matrix metalloproteinases (MMPs) inhibitory effects as the other two agents ( > 0.05), but much stronger antibacterial capability than 0.2% CHX ( < 0.05). This indicated that a cavity disinfectant with 5% DMADDM is promising for improving the stability of resin-dentin bonds in appearance of saliva biofilm; and the saliva microbial-aging method is more promising for studying the durability of resin-dentin bonds than water aging.
本研究旨在评估含5%甲基丙烯酸二甲氨基十二酯（DMADDM）的新型窝洞消毒剂的潜在益处；并比较唾液微生物老化法与水老化法在测量树脂-牙本质粘结强度变化方面的有效性。测试了三种窝洞消毒剂：0.2%氯己定（CHX）；5% DMADDM；以及5% DMADDM + 0.2% CHX。在唾液微生物老化或水老化一个月后进行微拉伸粘结强度（μTBS）测试。采用分光光度法测量胶原蛋白降解产物羟脯氨酸（HYP）。此外，评估了每种试剂的抗菌效果。在微生物老化一个月后，5% DMADDM导致的树脂-牙本质粘结强度损失百分比最小（<0.05）。在测试组中，水老化一个月后粘结强度下降无统计学显著差异（>0.05）。除5% DMADDM组外，在所有组中微生物老化法导致的粘结强度下降均比水老化法更多（<0.05）。同时，5% DMADDM与其他两种试剂具有相同的基质金属蛋白酶（MMPs）抑制作用（>0.05），但其抗菌能力比0.2% CHX强得多（<0.05）。这表明含5% DMADDM的窝洞消毒剂在唾液生物膜存在的情况下有望提高树脂-牙本质粘结的稳定性；并且唾液微生物老化法在研究树脂-牙本质粘结的耐久性方面比水老化法更具前景。
