Pereira Ildeu H L, Ayres Eliane, Averous Luc, Schlatter Guy, Hebraud Anne, de Paula Ana Cláudia Chagas, Viana Pedro Henrique Leroy, Goes Alfredo Miranda, Oréfice Rodrigo L
Department of Metallurgical and Materials Engineering, Federal University of Minas Gerais (UFMG), Pampulha, Belo Horizonte, MG, Brazil.
J Mater Sci Mater Med. 2014 Apr;25(4):1137-48. doi: 10.1007/s10856-013-5133-9. Epub 2013 Dec 31.
Mineralized poly(ε-caprolactone)/gelatin core-shell nanofibers were prepared via co-axial electrospinning and subsequent incubation in biomimetic simulated body fluid containing ten times the calcium and phosphate ion concentrations found in human blood plasma. The deposition of calcium phosphate on the nanofiber surfaces was investigated through scanning electronic microscopy and X-ray diffraction. Energy dispersive spectroscopy results indicated that calcium-deficient hydroxyapatite had grown on the fibers. Fourier transform infrared spectroscopy analysis suggested the presence of hydroxyl-carbonate-apatite. The results of a viability assay (MTT) and alkaline phosphatase activity analysis suggested that these mineralized matrices promote osteogenic differentiation of human adipose-derived stem cells (hASCs) when cultured in an osteogenic medium and have the potential to be used as a scaffold in bone tissue engineering. hASCs cultured in the presence of nanofibers in endothelial differentiation medium showed lower rates of proliferation than cells cultured without the nanofibers. However, endothelial cell markers were detected in cells cultured in the presence of nanofibers in endothelial differentiation medium.
通过同轴静电纺丝并随后在含有比人血浆中钙和磷酸根离子浓度高十倍的仿生模拟体液中孵育，制备了矿化聚（ε-己内酯）/明胶核壳纳米纤维。通过扫描电子显微镜和X射线衍射研究了纳米纤维表面磷酸钙的沉积情况。能量色散光谱结果表明，纤维上生长了缺钙羟基磷灰石。傅里叶变换红外光谱分析表明存在羟基碳酸磷灰石。活力测定（MTT）和碱性磷酸酶活性分析结果表明，这些矿化基质在成骨培养基中培养时可促进人脂肪来源干细胞（hASC）的成骨分化，并有潜力用作骨组织工程中的支架。在内皮分化培养基中，在纳米纤维存在下培养的hASC的增殖率低于无纳米纤维培养的细胞。然而，在内皮分化培养基中，在纳米纤维存在下培养的细胞中检测到了内皮细胞标记物。
