Liu Liyun, Lan Ruiting, Liu Liqin, Wang Yonglu, Zhang Yushi, Wang Yiting, Xu Jianguo
State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and PreventionBeijing, China.
Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesZhejiang, China.
Front Microbiol. 2017 Jul 20;8:1357. doi: 10.3389/fmicb.2017.01357. eCollection 2017.
spp. especially , is frequently causing nosocomial infections, and increasingly becoming multi-drug resistant (MDR). In this study, we aimed to determine the genetic diversity and relationships of spp. from diarrheal patients and food sources, their antimicrobial resistance profiles and virulence properties. Sixty two isolates, including 13 , 41 and eight isolates, were obtained from human diarrheal patients and food sources. Multilocus Sequence Typing (MLST) of seven housekeeping genes and antimicrobial susceptibility testing using the broth microdilution method according to CLSI recommendations were carried out. Adhesion and cytotoxicity to HEp-2 cells were performed. PCR and sequencing were used to identify , , and genes. The 62 isolates were divided into 53 sequence types (STs) with all STs being novel, displaying high genetic diversity. ST39 was a predominant ST shared by 5 strains isolated from four foods and a diarrheal patient. All isolates were resistant to cefoxitin, and sensitive to imipenem, meropenem and amikacin. The majority of isolates (61.3%) were MDR of three or more antibiotics out of the 22 antibiotics tested. Two isolates each carried the gene and a variant of . Three isolates each carried and genes. Seven isolates that showed strong cytotoxicity to HEp-2 cells were MDR. spp. from human and food sources are diverse with variation in virulence properties and antibiotic resistance profiles. Food may be an important source of species in transmission to humans. and are potential foodborne pathogens.
尤其是[具体菌名未给出]经常引起医院感染，并且越来越多地成为多重耐药菌（MDR）。在本研究中，我们旨在确定来自腹泻患者和食物源的[具体菌名未给出]的遗传多样性和关系、它们的抗菌药物耐药谱以及毒力特性。从人类腹泻患者和食物源中获得了62株分离株，包括13株[具体菌名未给出]、41株[具体菌名未给出]和8株[具体菌名未给出]分离株。对7个管家基因进行多位点序列分型（MLST），并根据CLSI建议使用肉汤微量稀释法进行抗菌药物敏感性测试。对HEp-2细胞进行黏附及细胞毒性检测。采用PCR和测序来鉴定[具体基因未给出]、[具体基因未给出]、[具体基因未给出]和[具体基因未给出]基因。这62株分离株被分为53个序列类型（STs），所有STs均为新发现的，显示出高度的遗传多样性。ST39是从四种食物和一名腹泻患者中分离出的5株菌株共有的主要ST。所有分离株对头孢西丁耐药，对亚胺培南、美罗培南和阿米卡星敏感。在所测试的22种抗生素中，大多数[具体菌名未给出]分离株（61.3%）对三种或更多种抗生素呈现多重耐药。两株[具体菌名未给出]分离株各自携带[具体基因未给出]基因和[具体基因未给出]的一个变体。三株[具体菌名未给出]分离株各自携带[具体基因未给出]和[具体基因未给出]基因。对HEp-2细胞显示出强细胞毒性的7株分离株为多重耐药菌。来自人类和食物源的[具体菌名未给出]具有多样性，其毒力特性和抗生素耐药谱存在差异。食物可能是[具体菌名未给出]物种传播给人类的重要来源。[具体菌名未给出]和[具体菌名未给出]是潜在的食源性病原体。
