Turrina Chiara, Berensmeier Sonja, Schwaminger Sebastian P
Bioseparation Engineering Group, Department of Mechanical Engineering, Technical University of Munich, 80333 München, Germany.
Pharmaceuticals (Basel). 2021 Apr 24;14(5):405. doi: 10.3390/ph14050405.
New drug delivery systems are a potential solution for administering drugs to reduce common side effects of traditional methods, such as in cancer therapy. Iron oxide nanoparticles (IONs) can increase the drugs' biological activity through high binding efficiency and magnetically targeted drug delivery. Understanding the adsorption and release process of a drug to the carrier material plays a significant role in research to generate an applicable and controlled drug delivery system. This contribution focuses on the binding patterns of the peptide lasioglossin III from bee venom on bare IONs. Lasioglossin has a high antimicrobial behavior and due to its cationic properties, it has high binding potential. Considering the influence of pH, the buffer type, the particle concentration, and time, the highest drug loading of 22.7% is achieved in phosphate-buffered saline. Analysis of the desorption conditions revealed temperature and salt concentration sensitivity. The nanoparticles and peptide-ION complexes are analyzed with dynamic light scattering, zeta potential, and infrared spectroscopy. Additionally, cytotoxicity experiments performed on show higher antimicrobial activity of bound lasioglossin than of the free peptide. Therefore, bare IONs are an interesting platform material for the development of drug-delivery carriers for cationic peptides.
新型药物递送系统是给药的一种潜在解决方案，可减少传统方法的常见副作用，如在癌症治疗中。氧化铁纳米颗粒（IONs）可通过高结合效率和磁靶向药物递送提高药物的生物活性。了解药物在载体材料上的吸附和释放过程对于开发适用的可控药物递送系统的研究具有重要作用。本文着重研究蜂毒中的肽类物质舌蜂毒肽III在裸露IONs上的结合模式。舌蜂毒肽具有较高的抗菌活性，因其阳离子特性，具有较高的结合潜力。考虑到pH值、缓冲液类型、颗粒浓度和时间的影响，在磷酸盐缓冲盐溶液中实现了22.7%的最高药物负载量。对解吸条件的分析揭示了温度和盐浓度敏感性。通过动态光散射、zeta电位和红外光谱对纳米颗粒和肽-ION复合物进行了分析。此外，对 进行的细胞毒性实验表明，结合态舌蜂毒肽的抗菌活性高于游离肽。因此，裸露的IONs是开发阳离子肽药物递送载体的一种有趣的平台材料。
