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铌掺杂羟基磷灰石生物陶瓷：合成、表征及细胞相容性

Niobium-Doped Hydroxyapatite Bioceramics: Synthesis, Characterization and Cytocompatibility.

作者信息

Capanema Nádia S V, Mansur Alexandra A P, Carvalho Sandhra M, Silva Alexandra R P, Ciminelli Virginia S, Mansur Herman S

机构信息

Center of Nanoscience, Nanotechnology and Innovation-CeNano2I, Department of Metallurgical and Materials Engineering, Federal University of Minas Gerais/UFMG, Av. Antônio Carlos, 6627 Escola de Engenharia, Belo Horizonte/MG 31.270-901, Brazil.

出版信息

Materials (Basel). 2015 Jul 9;8(7):4191-4209. doi: 10.3390/ma8074191.

DOI:10.3390/ma8074191
PMID:28793433
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5455653/
Abstract

Doping calcium phosphates with ionic species can play an important role in biological responses promoting alkaline phosphatase activity, and, therefore inducing the generation of new bone. Thus, in this study, the synthesis of niobium-doped hydroxyapatite (Nb-HA) nanosize particles obtained by the precipitation process in aqueous media followed by thermal treatment is presented. The bioceramics were extensively characterized by X-ray diffraction, wavelength dispersive X-ray fluorescence spectrometry, Fourier transform infrared spectroscopy, scanning electron microscopy/energy dispersive X-ray spectroscopy analysis, transmission electron microscopy, atomic force microscopy and thermal analysis regarding their chemical composition, structure and morphology. The results showed that the precipitate dried at 110 °C was composed of amorphous calcium phosphate and HA, with polidisperse particles ranging from micro to nano dimensions. After the thermal treatment at 900 °C, the bioceramic system evolved predominantly to HA crystalline phase, with evident features of particle sintering and reduction of surface area. Moreover, the addition of 10 mol% of niobium salt precursor during the synthesis indicated the complete incorporation of the Nb(V) species in the HA crystals with detectable changes in the original lattice parameters. Furthermore, the incorporation of Nb ions caused a significant refinement on the average particle size of HA. Finally, the preliminary cytocompatibility response of the biomaterials was accessed by human osteoblast cell culture using MTT and resazurin assays, which demonstrated no cytotoxicity of the Nb-alloyed hydroxyapatite. Thus, these findings seem promising for developing innovative Nb-doped calcium phosphates as artificial biomaterials for potential use in bone replacements and repair.

摘要

用离子物种掺杂磷酸钙在促进碱性磷酸酶活性从而诱导新骨生成的生物反应中可发挥重要作用。因此，在本研究中，介绍了通过水相沉淀法随后进行热处理获得的铌掺杂羟基磷灰石（Nb-HA）纳米尺寸颗粒的合成。通过X射线衍射、波长色散X射线荧光光谱法、傅里叶变换红外光谱法、扫描电子显微镜/能量色散X射线光谱分析、透射电子显微镜、原子力显微镜以及关于其化学成分、结构和形态的热分析对生物陶瓷进行了广泛表征。结果表明，在110°C下干燥的沉淀物由无定形磷酸钙和HA组成，颗粒多分散，尺寸范围从微米到纳米。在900°C热处理后，生物陶瓷体系主要演变为HA晶相，具有明显的颗粒烧结和表面积减小特征。此外，在合成过程中添加10 mol%的铌盐前驱体表明Nb(V)物种完全掺入HA晶体中，原始晶格参数发生了可检测的变化。此外，Nb离子的掺入导致HA平均粒径显著细化。最后，通过使用MTT和刃天青测定法的人成骨细胞培养来评估生物材料的初步细胞相容性反应，结果表明铌合金化羟基磷灰石没有细胞毒性。因此，这些发现对于开发创新的铌掺杂磷酸钙作为潜在用于骨替代和修复的人工生物材料似乎很有前景。

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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab28/5455653/d79b5db62121/materials-08-04191-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab28/5455653/8cdf4c8aab6a/materials-08-04191-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab28/5455653/216abaca108e/materials-08-04191-g009.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab28/5455653/0816e103effe/materials-08-04191-g012.jpg

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