Institute of Stomatology, Nanjing Medical University, 136 Hanzhong Road, Nanjing, 210029, Jiangsu, China.
Key Laboratory of Oral Diseases of Jiangsu Province and Stomatological Institute of Nanjing Medical University, 140 Hanzhong Road, Nanjing, 210029, Jiangsu, China.
Biomed Eng Online. 2020 Apr 3;19(1):18. doi: 10.1186/s12938-020-0756-9.
The remineralization approach mechanically occludes the exposed dentinal tubules mechanically, reduces the permeability of dentinal tubules and eliminates the symptoms of dentin hypersensitivity. The aim of the present study was to investigate the remineralization of demineralized dentin slices using CPP-ACP combined with TPP, and the research hypothesis was that CPP-ACP combined with TPP could result in extrafibrillar and intrafibrillar remineralization of dentin.
Demineralized dentin slices were prepared and randomly divided into the following groups: A (the CPP-ACP group), B (the CPP-ACP + TPP combination group), C (the artificial saliva group), D (the negative control group), and E (the positive control group). Dentin slice samples from groups A, B and C were remineralized and the remineralization effect was evaluated using scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy (EDX), attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) and X-ray diffraction (XRD).
Treatment with CPP-ACP combined with TPP occluded the dentinal tubules and resulted in remineralization of collagen fibrils. The hydroxyapatite crystals formed via remineralization were found to closely resemble the natural dentin components.
CPP-ACP combined with TPP has a good remineralization effect on demineralized dentin slices.
再矿化方法通过机械方式封闭暴露的牙本质小管，降低牙本质小管的渗透性，并消除牙本质敏感症状。本研究旨在探讨 CPP-ACP 联合 TPP 对脱矿牙本质片的再矿化作用，研究假设是 CPP-ACP 联合 TPP 可以导致牙本质的纤维外和纤维内再矿化。
制备脱矿牙本质片，并随机分为以下组：A（CPP-ACP 组）、B（CPP-ACP+TPP 联合组）、C（人工唾液组）、D（阴性对照组）和 E（阳性对照组）。对 A、B 和 C 组的牙本质片样本进行再矿化处理，并用扫描电子显微镜（SEM）、透射电子显微镜（TEM）、能谱分析（EDX）、衰减全反射傅里叶变换红外光谱（ATR-FTIR）和 X 射线衍射（XRD）评估再矿化效果。
CPP-ACP 联合 TPP 处理封闭了牙本质小管，并导致胶原纤维的再矿化。形成的羟基磷灰石晶体与天然牙本质成分非常相似。
CPP-ACP 联合 TPP 对脱矿牙本质片有良好的再矿化效果。
