使用位点特异性探针通过限制性片段长度多态性（RFLP）检测到的HLA - B27和其他B位点抗原的新多态性。
New polymorphisms of HLA-B27 and other B locus antigens detected by RFLP using a locus-specific probe.
作者信息
Ness D B, Grumet F C
出版信息
Hum Immunol. 1987 Jan;18(1):65-73. doi: 10.1016/0198-8859(87)90113-3.
Genomic DNA from 46 B27+ ankylosing spondylitis, Reiter's syndrome, or normal individuals was digested with Taq I and probed, in Southern blots, with the HLA-B locus specific probe, EI7. Four restriction fragment length polymorphisms (RFLP), 2.5, 3.4, 3.8 and 4.0 or 8.0 kb, were observed for the B27 gene. In Caucasians, one of the B27 variants (2.5 kb) was more frequent in normals and almost never appeared in patients, suggesting a trend that is not yet statistically significant. In the course of defining the B27 polymorphisms, three and two RFLP, respectively, were also found for the B18 and B44 genes.
从46名B27阳性的强直性脊柱炎、赖特综合征患者或正常个体中提取基因组DNA，用Taq I进行消化，并在Southern印迹中用HLA - B基因座特异性探针EI7进行检测。观察到B27基因有四种限制性片段长度多态性（RFLP），分别为2.5、3.4、3.8和4.0或8.0 kb。在白种人中，B27的一种变体（2.5 kb）在正常个体中更为常见，在患者中几乎从未出现，这表明一种趋势，但尚未具有统计学意义。在确定B27多态性的过程中，还分别发现B18和B44基因有三种和两种RFLP。
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