来自肺炎链球菌的含磷壁酸的胞壁肽作为肺炎球菌自溶素的底物。
Teichoic acid-containing muropeptides from Streptococcus pneumoniae as substrates for the pneumococcal autolysin.
作者信息
Garcia-Bustos J F, Tomasz A
出版信息
J Bacteriol. 1987 Feb;169(2):447-53. doi: 10.1128/jb.169.2.447-453.1987.
Abstract
Pneumococcal cell walls in which the normal phosphorylcholine component of the wall teichoic acids is replaced with phosphorylethanolamine cannot absorb the homologous autolytic enzyme and are completely resistant to autolytic degradation (S. Giudicelli and A. Tomasz, J. Bacteriol. 158:1188-1190, 1984). We have now isolated and characterized soluble teichoic acid-containing muropeptides from such cell walls and tested them as substrates for the pneumococcal autolytic enzyme. Both choline- and ethanolamine-containing muropeptides were hydrolyzed to the same extent by the enzyme. Furthermore, free choline concentrations that totally inhibited the digestion of pneumococcal cell walls in vivo and in vitro were without effect when the soluble substrates were used.
摘要
在肺炎球菌细胞壁中，壁磷壁酸的正常磷酰胆碱成分被磷酰乙醇胺取代后，无法吸收同源自溶酶，并且对自溶降解完全具有抗性（S. 朱迪塞利和A. 托马兹，《细菌学杂志》158:1188 - 1190，1984年）。我们现在已经从这类细胞壁中分离并鉴定了含可溶性磷壁酸的胞壁肽，并将它们作为肺炎球菌自溶酶的底物进行测试。含胆碱和含乙醇胺的胞壁肽被该酶水解的程度相同。此外，在体内和体外完全抑制肺炎球菌细胞壁消化的游离胆碱浓度，在使用可溶性底物时则没有效果。
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本文引用的文献
1
Structure of the complex polysaccharide C-substance from Streptococcus pneumoniae type 1.1型肺炎链球菌复合多糖C物质的结构
Biochemistry. 1980 Sep 30;19(20):4712-9. doi: 10.1021/bi00561a026.
2
Periplasmic protein associated with the oligopeptide permeases of Salmonella typhimurium and Escherichia coli.与鼠伤寒沙门氏菌和大肠杆菌的寡肽通透酶相关的周质蛋白。
J Bacteriol. 1983 Sep;155(3):1434-8. doi: 10.1128/jb.155.3.1434-1438.1983.
3
Attachment of pneumococcal autolysin to wall teichoic acids, an essential step in enzymatic wall degradation.肺炎球菌自溶素与壁磷壁酸的附着，这是酶促细胞壁降解的关键步骤。
J Bacteriol. 1984 Jun;158(3):1188-90. doi: 10.1128/jb.158.3.1188-1190.1984.
4
Effect of alanine ester substitution and other structural features of lipoteichoic acids on their inhibitory activity against autolysins of Staphylococcus aureus.丙氨酸酯取代及脂磷壁酸的其他结构特征对其抑制金黄色葡萄球菌自溶素活性的影响。
J Bacteriol. 1981 May;146(2):467-75. doi: 10.1128/jb.146.2.467-475.1981.
5
Affinity labeling of a phosphorylcholine binding mouse myeloma protein.磷酸胆碱结合小鼠骨髓瘤蛋白的亲和标记
Biochemistry. 1972 Feb 29;11(5):766-71. doi: 10.1021/bi00755a014.
6
Regulation of the bacterial cell wall: analysis of a mutant of Bacillus subtilis defective in biosynthesis of teichoic acid.细菌细胞壁的调控：枯草芽孢杆菌磷壁酸生物合成缺陷突变体的分析
J Bacteriol. 1972 Apr;110(1):281-90. doi: 10.1128/jb.110.1.281-290.1972.
7
Abnormal autolytic enzyme in a pneumococus with altered teichoic acid composition.具有改变的磷壁酸组成的肺炎球菌中的异常自溶酶。
Proc Natl Acad Sci U S A. 1971 Nov;68(11):2627-30. doi: 10.1073/pnas.68.11.2627.
8
Multiple antibiotic resistance in a bacterium with suppressed autolytic system.具有受抑制自溶系统的细菌中的多重抗生素耐药性
Nature. 1970 Jul 11;227(5254):138-40. doi: 10.1038/227138a0.
9
10
Pneumococcal C-substance, a ribitol teichoic acid containing choline phosphate.肺炎球菌C物质，一种含磷酸胆碱的核糖醇壁酸。
Biochem J. 1968 Dec;110(3):573-82. doi: 10.1042/bj1100573.
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