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表皮葡萄球菌生物膜定量：不同溶剂和染料的影响。

Staphylococcus epidermidis biofilm quantification: effect of different solvents and dyes.

机构信息

Institute for Risk Assessment Sciences, Division Veterinary Pharmacology, Pharmacotherapy and Toxicology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.

Institute for Risk Assessment Sciences, Division Veterinary Pharmacology, Pharmacotherapy and Toxicology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.

出版信息

J Microbiol Methods. 2014 Jun;101:63-6. doi: 10.1016/j.mimet.2014.03.016. Epub 2014 Apr 12.

DOI:10.1016/j.mimet.2014.03.016
PMID:24726871
Abstract

Staphylococcus epidermidis biofilm formed in the presence of the solvents DMSO, ethanol or methanol was quantified using safranin or crystal violet staining protocols. We found that biofilm quantification was the most accurate when safranin protocol was applied. Moreover, both DMSO and ethanol stimulated biofilm formation.

摘要

采用吖啶橙或结晶紫染色方案对含二甲基亚砜（DMSO）、乙醇或甲醇溶剂存在条件下形成的表皮葡萄球菌生物膜进行定量分析。我们发现，应用吖啶橙方案时生物膜定量最为准确。此外，DMSO 和乙醇均能刺激生物膜形成。

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