Key Laboratory of Integrated Regulation and Resources Development, College of Environment, Hohai University, Nanjing, 210098, China.
Key Laboratory of Integrated Regulation and Resources Development, College of Environment, Hohai University, Nanjing, 210098, China; Wentian College, Hohai University, Ma'anshan, 243031, China.
Environ Toxicol Pharmacol. 2017 Oct;55:14-19. doi: 10.1016/j.etap.2017.08.002. Epub 2017 Aug 7.
The presence of pharmaceuticals in the aquatic environment has received great attention due to their potential impacts on public health. The single, as well as the combined toxicities of erythromycin (ERY) and ketoconazole (KCZ) on the bioaccumulation, biochemical and behavioral responses, were examined in crucian carp. This study focused on the uptake of contaminants, acetylcholinesterase (AChE) activity in the brain, swimming and shoaling behavior of fish. After 14days of binary exposure, the addition of KCZ at nominal concentrations of 0.2, 2 and 20μg/L significantly increased the accumulation of ERY in the brain of the fish and the bioconcentration factor of 2.08 was 2.6-fold higher than that calculated from the ERY-alone exposure. The brain AChE activity was significantly inhibited by ERY and KCZ with a significant correlation with respect to the accumulative concentration of the contaminants. The inhibition rates of swimming activity to KCZ were increased with a corresponding increase in the exposure concentration of KCZ in the single exposure. However, this manner was altered by the combined exposure. In addition, shoaling was significantly enhanced by KCZ-alone exposure, which was significantly correlated with the swimming activity. This study indicates that the mixture of the contaminants may cause endocrine disrupting effects and behavior modification especially in fish with known ecological and evolutionary consequences.
由于药品对公众健康的潜在影响，其在水生环境中的存在受到了极大关注。本研究以鲫鱼为实验对象，研究了单一及联合使用红霉素（ERY）和酮康唑（KCZ）对生物蓄积、生化和行为反应的毒性作用。本研究重点关注污染物的摄取、脑乙酰胆碱酯酶（AChE）活性、鱼类的游泳和集群行为。在二元暴露 14 天后，在名义浓度为 0.2、2 和 20μg/L 的 KCZ 的添加下，显著增加了 ERY 在鱼脑中的积累，其生物浓缩因子比单独暴露于 ERY 时的计算值高 2.6 倍。ERY 和 KCZ 显著抑制了脑 AChE 活性，与污染物的累积浓度有显著相关性。单一暴露时，游泳活性对 KCZ 的抑制率随着 KCZ 暴露浓度的相应增加而增加，但这种方式在联合暴露时发生了改变。此外，KCZ 单一暴露显著增强了集群行为，这与游泳活性显著相关。本研究表明，污染物的混合物可能会导致内分泌干扰效应和行为改变，特别是对具有已知生态和进化后果的鱼类。
