Brown-Elliott Barbara A, Simmer Patricia J, Trovato Alberto, Hyle Emily P, Droz Sara, Buckwalter Seanne P, Borroni Emanuele, Branda John A, Iana Elkina, Mariottini Alessandro, Nelson Jameelah, Matteelli Alberto, Toney Nadege C, Scarparo Claudio, de Man Tom J B, Vasireddy Ravikiran, Gandhi Rajesh T, Wengenack Nancy L, Cirillo Daniela M, Wallace Richard J, Tortoli Enrico
1Department of Microbiology, The University of Texas Health Science Center at Tyler, Tyler, Texas, USA.
2Division of Medical Microbiology, Johns Hopkins Medical Institute, Baltimore, Maryland, USA.
Int J Syst Evol Microbiol. 2018 Nov;68(11):3557-3562. doi: 10.1099/ijsem.0.003031. Epub 2018 Sep 11.
Two mycobacterial strains with close similarity to the Mycobacterium tuberculosis complex (MTBC) were isolated from cutaneous lesions of patients in the USA and Italy. At the phenotypic level, similarities to the MTBC included slow growth rate, rough morphotype of the unpigmented colonies and nearly identical high-performance liquid chromatography profiles of mycolic acids. In contrast to the MTBC, the strains were niacin- and nitrate-negative, and catalase-positive both at 68 °C and in semi-quantitative tests. The clinical isolates were more closely related to M. tuberculosis than to any other known mycobacterium and scored positive with commercial DNA probes (Hologic AccuProbe M. tuberculosis). Both average nucleotide identity and genome-to-genome distance suggested the strains are different from the MTBC. Therefore, given the distinguishing phenotypic and genomic-scale differences, we submit that the strains belong to a new species we have named Mycobacteriumdecipiens with type strain TBL 1200985 (=ATCC TSD-117=DSM 105360).
从美国和意大利患者的皮肤损伤中分离出了两种与结核分枝杆菌复合群（MTBC）极为相似的分枝杆菌菌株。在表型水平上，与MTBC的相似之处包括生长速度缓慢、无色菌落的粗糙形态型以及几乎相同的分枝菌酸高效液相色谱图谱。与MTBC不同的是，这些菌株烟酸和硝酸盐呈阴性，在68°C和半定量试验中过氧化氢酶均呈阳性。临床分离株与结核分枝杆菌的亲缘关系比与任何其他已知分枝杆菌的亲缘关系更近，并且用商业DNA探针（Hologic AccuProbe结核分枝杆菌）检测呈阳性。平均核苷酸同一性和全基因组距离均表明这些菌株与MTBC不同。因此，鉴于其显著的表型和基因组规模差异，我们认为这些菌株属于一个新物种，我们将其命名为隐匿分枝杆菌，模式菌株为TBL 1200985（=ATCC TSD-117=DSM 105360）。
