Department of Plant Resources, College of Industrial Sciences, Kongju National University, Yesan, 32439, Republic of Korea.
State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, 230036, People's Republic of China.
Mol Genet Genomics. 2017 Dec;292(6):1391-1403. doi: 10.1007/s00438-017-1354-9. Epub 2017 Aug 18.
Salt is the major factor limiting crop productivity in saline soils and is controlled by various genes. The development of salt-tolerant rice through molecular breeding methods is important to meet the needs of rice breeding. We used 295 accessions to perform a genome-wide association study (GWAS) of salt tolerance-related phenotypes in rice at the seedling stage and obtained 93 candidate genes with high association peaks across six phenotypes. We constructed a protein interaction network using the candidate genes identified here, and 33 genes were associated. Based on the expression patterns, we found that most of these genes showed a different expression level under control and salt stress conditions. In addition, haplotype network and sequence analysis of one 'key' gene, a transcription factor (Os12g0176700) encoding a SWIRM domain-containing protein, in the interaction network was investigated to explore its possible role in the network. Our study revealed candidate salt tolerance-related genes in rice at the seedling stage, and demonstrated the feasibility of using GWAS to identify genetic architecture underlying salt tolerance. The data generated here may provide resources for molecular breeding and functional analysis of salt tolerance in rice seedlings.
盐是限制盐渍土中作物生产力的主要因素，受多种基因控制。通过分子育种方法培育耐盐水稻对满足水稻育种的需求非常重要。我们使用 295 个品系对水稻幼苗期的耐盐相关表型进行了全基因组关联研究（GWAS），在六个表型中获得了 93 个具有高关联峰的候选基因。我们使用这里鉴定的候选基因构建了一个蛋白质相互作用网络，有 33 个基因与之相关。根据表达模式，我们发现这些基因中的大多数在对照和盐胁迫条件下表现出不同的表达水平。此外，我们还研究了互作网络中一个“关键”基因（编码一个具有 SWIRM 结构域的转录因子的 Os12g0176700）的单倍型网络和序列分析，以探讨其在网络中的可能作用。我们的研究揭示了水稻幼苗期与耐盐性相关的候选基因，证明了使用 GWAS 来鉴定耐盐性遗传结构的可行性。这里生成的数据可能为水稻幼苗耐盐性的分子育种和功能分析提供资源。
