Borisjuk Ljudmilla, Rolletschek Hardy, Fuchs Johannes, Melkus Gerd, Neuberger Thomas
Leibniz-Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, Gatersleben 06466, Germany.
Department of Experimental Physics 5 (Biophysics), University of Würzburg, Am Hubland, Würzburg D-97074, Germany.
Materials (Basel). 2011 Aug 16;4(8):1426-1439. doi: 10.3390/ma4081426.
Low field NMR has been successfully used for the evaluation of seed composition and quality, but largely only in crop species. We show here that 1.5T NMR provides a reliable means for analysing the seed lipid fraction present in a wide range of species, where both the seed size and lipid concentration differed by >10 fold. Little use of high field NMR has been made in seed research to date, even though it potentially offers many opportunities for studying seed development, metabolism and storage. Here we demonstrate how 17.5T and 20T NMR can be applied to image seed structure, and analyse lipid and metabolite distribution. We suggest that further technical developments in NMR/MRI will facilitate significant advances in our understanding of seed biology.
低场核磁共振已成功用于评估种子成分和质量，但主要仅用于农作物物种。我们在此表明，1.5T核磁共振为分析广泛物种中存在的种子脂质部分提供了一种可靠的方法，这些物种的种子大小和脂质浓度差异超过10倍。尽管高场核磁共振在研究种子发育、代谢和储存方面可能提供许多机会，但迄今为止在种子研究中很少使用。在此我们展示了17.5T和20T核磁共振如何用于对种子结构进行成像，并分析脂质和代谢物分布。我们认为，核磁共振/磁共振成像技术的进一步发展将有助于我们在种子生物学理解方面取得重大进展。
