Cacho Nicole T, Harrison Natalie A, Parker Leslie A, Padgett Kaylie A, Lemas Dominick J, Marcial Guillermo E, Li Nan, Carr Laura E, Neu Josef, Lorca Graciela L
Division of Neonatology, Department of Pediatrics, College of Medicine, University of Florida, GainesvilleFL, United States.
Department of Microbiology and Cell Science, Genetics Institute, Institute of Food and Agricultural Sciences, University of Florida, GainesvilleFL, United States.
Front Microbiol. 2017 Aug 3;8:1470. doi: 10.3389/fmicb.2017.01470. eCollection 2017.
The American Academy of Pediatrics recommends that extremely preterm infants receive mother's own milk (MOM) when available or pasteurized donor breast milk (DBM) when MOM is unavailable. The goal of this study was to determine whether DBM could be inoculated with MOM from mothers of preterm infants to restore the live microbiota (RM). Culture dependent and culture independent methods were used to analyze the fluctuations in the overall population and microbiome, respectively, of DBM, MOM, and RM samples over time. Using MOM at time = 0 (T0) as the target for the restoration process, this level was reached in the 10% (RM-10) and 30% (RM-30) mixtures after 4 h of incubation at 37°C, whereas, the larger dilutions of 1% (RM-1) and 5% (RM-5) after 8 h. The diversity indexes were similar between MOM and DBM samples, however, different genera were prevalent in each group. Interestingly, 40% of the bacterial families were able to expand in DBM after 4 h of incubation indicating that a large percentage of the bacterial load present in MOM can grow when transferred to DBM, however, no core microbiome was identified. In summary, the microbiome analyses indicated that each mother has a unique microbiota and that live microbial reestablishment of DBM may provide these microbes to individual mothers' infants. The agreement between the results obtained from the viable bacterial counts and the microbiome analyses indicate that DBM incubated with 10-30% v/v of the MOM for 4 h is a reasonable restoration strategy.
美国儿科学会建议，极早产儿在有条件时应接受母亲自身的母乳（MOM），若无母亲自身的母乳，则应接受巴氏消毒的捐赠母乳（DBM）。本研究的目的是确定是否可以用早产儿母亲的MOM接种DBM，以恢复其活微生物群（RM）。分别使用依赖培养和不依赖培养的方法，分析DBM、MOM和RM样本随时间推移在总体菌群和微生物组方面的波动情况。以时间=0（T0）时的MOM作为恢复过程的目标，在37℃孵育4小时后，10%（RM-10）和30%（RM-30）的混合物达到了该水平，而1%（RM-1）和5%（RM-5）的较大稀释度在8小时后达到该水平。MOM和DBM样本的多样性指数相似，然而，每组中普遍存在的属不同。有趣的是，孵育4小时后，40%的细菌科能够在DBM中扩增，这表明MOM中存在的很大一部分细菌负荷转移到DBM后能够生长，然而，未鉴定出核心微生物组。总之，微生物组分析表明，每位母亲都有独特的微生物群，DBM的活微生物重建可能会将这些微生物提供给个别母亲的婴儿。活菌计数结果与微生物组分析结果之间的一致性表明，用10%-30%（v/v）的MOM孵育DBM 4小时是一种合理的恢复策略。
