Kim Jisu, Park Jonghoon, Kim Bokyung, Lee Chi-Ho, Lim Kiwon, Suh Heajung
Physical Activity and Performance Institute (PAPI), Konkuk University, Seoul, Republic of Korea.
Department of Physical Education, Korea University, Seoul, Republic of Korea.
J Exerc Nutrition Biochem. 2017 Mar 31;21(1):21-25. doi: 10.20463/jenb.2017.0056.
This study was carried out to determine the optimal dose of silk peptide for enhancing fat metabolism during exercise.
Fifty male ICR mice were randomly divided into five groups: Sed, SP0, SP200, SP400, and SP800. All SP mice underwent training by running on a treadmill 5 times a week for 2 weeks (20 m/min, 8° slope, 50 min/day for the first week and 25 m/min, 8° slope, 50 min/day at about 70-75% of maximum oxygen uptake for the second week).
After the 2 weeks, fat oxidation was measured during a 1-h exercise at the training conditions of the second week and was found to be 1.02 ± 0.15, 1.04 ± 0.17, 0.98 ± 0.10, 1.14 ± 0.19, and 1.15 ± 0.07 g/kg/h for Sed, SP0, SP200, SP400, and SP800 groups, respectively. The SP800 group had significantly higher fat oxidation levels than the SP0 group did at 36, 40, and 56 min and the Sed group did at 2, 4, 6, 8, 12, 14, 16, 20, 40, 46, 50, 52, 56, and 60 min. However, there was no significant difference among the groups in carbohydrate oxidation during the 1-h exercise. SP doses of 200 mg/kg and 400 mg/kg did not show any effect on fat and carbohydrate oxidation.
In conclusion, 800 mg/kg of silk peptide is the optimal dose for enhancing fat metabolism during exercise. In addition, silk peptide treatment could reduce body weight by enhancing fat metabolism.
本研究旨在确定运动期间增强脂肪代谢的丝肽最佳剂量。
将50只雄性ICR小鼠随机分为五组：对照组（Sed）、丝肽0组（SP0）、丝肽200组（SP200）、丝肽400组（SP400）和丝肽800组（SP800）。所有丝肽组小鼠每周在跑步机上训练5次，持续2周（第一周速度为20米/分钟，坡度为8°，每天50分钟；第二周速度为25米/分钟，坡度为8°，每天50分钟，约为最大摄氧量的70 - 75%）。
2周后，在第二周训练条件下进行1小时运动期间测量脂肪氧化，发现对照组、丝肽0组、丝肽200组、丝肽400组和丝肽800组的脂肪氧化量分别为1.02±0.15、1.04±0.17、0.98±0.10、1.14±0.19和1.15±0.07克/千克/小时。丝肽800组在运动36、40和56分钟时的脂肪氧化水平显著高于丝肽0组，在运动2、4、6、8、12、14、16、20、40、46、50、52、56和60分钟时显著高于对照组。然而，在1小时运动期间，各组间碳水化合物氧化无显著差异。200毫克/千克和400毫克/千克的丝肽剂量对脂肪和碳水化合物氧化无任何影响。
总之，800毫克/千克的丝肽是运动期间增强脂肪代谢的最佳剂量。此外，丝肽治疗可通过增强脂肪代谢减轻体重。
