Sidhu Chandni, Thakur Suresh, Sharma Gunjan, Tanuku Naga Radha Srinivas, Pinnaka Anil Kumar
MTCC-Microbial Type Culture Collection and Gene Bank, CSIR-Institute of Microbial Technology, Chandigarh-160036, India.
CSIR-National Institute of Oceanography, Regional Centre, 176, Lawsons Bay Colony, Visakhapatnam-530017, India.
Int J Syst Evol Microbiol. 2017 Sep;67(9):3428-3434. doi: 10.1099/ijsem.0.002132. Epub 2017 Aug 22.
A novel Gram-staining-negative, spiral-shaped, pale-yellow, non-sporulating, motile, aerobic bacterium, designated strain AK56T, was isolated from a sediment sample collected at the Coringa Wildlife Sanctuary, India. Colonies on marine agar were circular, pale yellow, shiny, translucent, 1-2 mm in diameter, convex and had an entire margin. The major fatty acids included C16 : 1, C16 : 1ω7c/C16 : 1ω6c and C18 : 1ω7c. Polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids, one unidentified phospholipid and five unidentified lipids. DNA-DNA hybridization between strain AK56T and Oceanospirillum linum LMG 5214T and 'Oceanospirillum nioense ' NIO-S6 showed relatedness values of 39.91 and 23.62 %, respectively. The DNA G+C content of strain AK56T was found to be 50.3 mol%. A sequence similarity search for the 16S rRNA gene sequence revealed that O. linum and O. nioense were the nearest phylogenetic neighbours, with a pair-wise sequence similarity of 98.9 and 98.2 %, respectively. Phylogenetic analysis also showed the formation of a cluster including strain AK56T with close relative O. linum and O. nioense. Based on the observed phenotypic, chemotaxonomic characteristics and phylogenetic analysis, strain AK56T is described in this study as a novel species in the genus Oceanospirillum, for which the name Oceanospirillum sanctuarii sp. nov. is proposed. The type strain of Oceanospirillumsanctuarii is AK56T (=MTCC 12005T=JCM 19193T=KCTC 52973T).
从印度科林加野生动物保护区采集的一份沉积物样本中分离出一株新型革兰氏染色阴性、螺旋状、淡黄色、无芽孢、具运动性的需氧细菌，命名为菌株AK56T。该菌株在海洋琼脂上的菌落呈圆形，淡黄色，有光泽，半透明，直径1-2毫米，凸起，边缘整齐。主要脂肪酸包括C16 : 1、C16 : 1ω7c/C16 : 1ω6c和C18 : 1ω7c。极性脂质包括双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、两种未鉴定的氨基脂质、一种未鉴定的磷脂和五种未鉴定的脂质。菌株AK56T与亚麻海螺旋菌LMG 5214T和“nioense海螺旋菌”NIO-S6之间的DNA-DNA杂交相关性值分别为39.91%和23.62%。发现菌株AK56T的DNA G+C含量为50.3摩尔%。对16S rRNA基因序列进行的序列相似性搜索显示，亚麻海螺旋菌和nioense海螺旋菌是最近的系统发育邻居，成对序列相似性分别为98.9%和98.2%。系统发育分析还表明，包括菌株AK56T及其近缘种亚麻海螺旋菌和nioense海螺旋菌在内形成了一个聚类。基于观察到的表型、化学分类特征和系统发育分析，本研究将菌株AK56T描述为海螺旋菌属中的一个新物种，提议将其命名为Oceanospirillum sanctuarii sp. nov.。Oceanospirillum sanctuarii的模式菌株为AK56T（=MTCC 12005T=JCM 19193T=KCTC 52973T）。
