MTCC-Microbial Type Culture Collection and Gene Bank, CSIR-Institute of Microbial Technology, Chandigarh, 160036, India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India.
Antonie Van Leeuwenhoek. 2021 Jul;114(7):1117-1130. doi: 10.1007/s10482-021-01582-y. Epub 2021 Jun 1.
A novel Gram-staining-negative, rod-shaped, 0.6-0.8 µm wide and 2.0-3.0 µm in length, motile bacterium designated strain AK62, was isolated from the green algal mat collected from saltpan, Kakinada, Andhra Pradesh, India. Colonies on ZMA were circular, off-white, shiny, moist, translucent, 1-2 mm in diameter, flat, with an entire margin. The major fatty acids include C, C ω7c, and summed feature 3 (C ω7c and/or C ω6c and/or iso-C 3-OH). Polar lipids include diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminophospholipid, three unidentified phospholipids, and one unidentified lipid. Polyamine includes Spermidine. The DNA G + C content of the strain AK62 was 58.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain AK62 was closely related to the type strains Marinobacterium sediminicola, Marinobacterium coralli and Marinobacterium stanieri with a pair-wise sequence similarity of 96.9, 96.6 and 96.6%, respectively, forming a distinct branch within the genus Marinobacterium and clustered with M. stanieri, M. sediminicola, M. coralli and M. maritimum cluster. Strain AK62 shares average nucleotide identity (ANIb, based on BLAST) of 78.44, 76.69, and 76.95% with M. sediminicola CGMCC 1.7287, M. stanieri DSM 7027, and Marinobacterium halophilum Mano11 respectively. Based on the observed phenotypic, chemotaxonomic characteristics, and phylogenetic analysis, strain AK62 is described in this study as a novel species in the genus Marinobacterium, for which the name Marinobacterium alkalitolerans sp. nov. is proposed. The type strain of M. alkalitolerans is AK62 (= MTCC 12102 = JCM 31159 = KCTC 52667).
一株新型革兰氏阴性、杆状、宽 0.6-0.8μm、长 2.0-3.0μm、可运动的细菌，菌株 AK62，从印度安得拉邦卡基纳达盐田的绿藻垫中分离得到。在 ZMA 上的菌落为圆形、灰白色、有光泽、湿润、半透明，直径 1-2mm，平坦，边缘完整。主要脂肪酸包括 C 16:0、C 17:1ω7c 和特征性脂肪酸 3（C 17:1ω7c 和/或 C 16:1ω7c 和/或 iso-C 15:0 3-OH）。极性脂包括双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、一种未鉴定的氨基磷脂、三种未鉴定的磷脂和一种未鉴定的脂类。多胺包括亚精胺。菌株 AK62 的 DNA G+C 含量为 58.8mol%。基于 16S rRNA 基因序列的系统发育分析表明，菌株 AK62 与模式菌株 Marinobacterium sediminicola、Marinobacterium coralli 和 Marinobacterium stanieri 的亲缘关系最近，两两之间的序列相似性分别为 96.9%、96.6%和 96.6%，在 Marinobacterium 属内形成一个独特的分支，并与 M. stanieri、M. sediminicola、M. coralli 和 M. maritimum 聚类聚集在一起。菌株 AK62 与 Marinobacterium sediminicola CGMCC 1.7287、Marinobacterium stanieri DSM 7027 和 Marinobacterium halophilum Mano11 的平均核苷酸同一性（ANIb，基于 BLAST）分别为 78.44%、76.69%和 76.95%。根据观察到的表型、化学分类特征和系统发育分析，本研究将菌株 AK62 描述为 Marinobacterium 属的一个新种，命名为 Marinobacterium alkalitolerans sp. nov.。M. alkalitolerans 的模式菌株为 AK62（= MTCC 12102= JCM 31159= KCTC 52667）。
