School of Basic Medical Sciences, Binzhou Medical University, Yantai, China.
College of Marine Science, Shandong University, Weihai, China.
Curr Microbiol. 2022 Mar 8;79(4):124. doi: 10.1007/s00284-022-02816-6.
A novel Gram-negative, motile, aerobic, spiral-shaped bacterium designated D5, was isolated from a coastal sediment collected in the Yellow Sea. Optimal growth occurred at 30 °C, pH 7.0-8.0 and in the presence of 1-3% (w/v) NaCl. Strain D5 contained ubiquinone 8 (Q-8) as the predominant respiratory quinone. The major fatty acids (> 10%) were C, C ω7c/C ω6c and Cw7c/Cw6c. The main polar lipids were phosphatidylglycerol and phosphatidylethanolamine. The draft genome is 5.6 Mb in length, and DNA G + C content is 47.2 mol%. 16S rRNA gene sequences showed that strain D5 is most closely related to Oceanospirillum beijerinckii NBRC 15445 (97.8%, sequence similarity). However, the digital DNA-DNA hybridization (dDDH) value and average nucleotide identity (ANI) between strain D5 and O. beijerinckii is only 27.8% and 77.1%. Phylogenetic trees based on 16S rRNA gene sequences and whole genomes all indicated that strain D5 formed a separate branch in the genus Oceanospirillum. Combined results of the polyphasic analyses suggested that strain D5 represents a novel species in the genus Oceanospirillum, for which the name Oceanospirillum sediminis sp. nov. is proposed. The type strain is D5 (= MCCC 1K06061 = KCTC 62987).
一株新的革兰氏阴性、能动、需氧、螺旋形细菌，命名为 D5，从黄海沉积物中分离得到。最佳生长条件为 30°C、pH7.0-8.0 和 1-3%（w/v）NaCl。菌株 D5 含有泛醌 8（Q-8）作为主要的呼吸醌。主要脂肪酸（>10%）为 C、Cω7c/Cω6c 和 Cw7c/Cw6c。主要极性脂为磷脂酰甘油和磷脂酰乙醇胺。基因组草图长 5.6 Mb，DNA G+C 含量为 47.2 mol%。16S rRNA 基因序列显示，菌株 D5 与海洋螺旋菌 NBRC 15445（97.8%，序列相似性）最为密切相关。然而，菌株 D5 和 O. beijerinckii 之间的数字 DNA-DNA 杂交（dDDH）值和平均核苷酸同一性（ANI）仅为 27.8%和 77.1%。基于 16S rRNA 基因序列和全基因组构建的系统发育树均表明，菌株 D5 在海洋螺旋菌属中形成了一个单独的分支。多相分析的综合结果表明，菌株 D5 代表海洋螺旋菌属中的一个新种，建议将其命名为 Sediminis 海洋螺旋菌 sp. nov。模式菌株为 D5（=MCCC 1K06061=KCTC 62987）。
