CSIR-National Institute of Oceanography, Regional Centre, P B No. 1913, Dr. Salim Ali Road, Kochi, 682018, Kerala, India.
Antonie Van Leeuwenhoek. 2013 May;103(5):1015-21. doi: 10.1007/s10482-013-9881-9. Epub 2013 Jan 22.
A novel Gram-negative, spiral shaped, motile bacterium, designated strain NIO-S6(T), was isolated from a sediment sample collected from Off-shore Rameswaram, Tamilnadu, India. Strain NIO-S6(T) was found to be positive for oxidase, DNase and lysine decarboxylase activities and negative for catalase, gelatinase, lipase, ornithine decarboxylase, nitrate reductase, aesculinase, amylase and urease activities. The fatty acids were determined to be dominated by C10:0 3OH, C16:0, C16:1 and C18:1. Strain NIO-S6(T) contains Q-8 as the major respiratory quinone. The DNA G+C content of the strain NIO-S6(T) was determined to be 49.5 ± 0.6 mol %. Phylogenetic analysis based on 16S rRNA gene sequence of strain NIO-S6(T) indicated Oceanospirillum linum and Oceanospirillum maris of the family Oceanospirillaceae (phylum Proteobacteria) are the closest related species with sequence similarities of 98.4 and 97.8 % respectively. Other members of the family showed sequence similarities <96.4 %. However, DNA-DNA hybridization with Oceanospirillum linum LMG 5214(T) and Oceanospirillum maris LMG 5213(T) showed a relatedness of 31.5 and 46.9 % with respect to strain NIO-S6(T). Based on the phenotypic characteristics and on phylogenetic inference, strain NIO-S6(T) is proposed as a novel species of the genus Oceanospirillum as Oceanospirillum nioense sp. nov. and the type strain is NIO-S6(T) (=MTCC 11154(T) = KCTC 32008(T)).
一株新的革兰氏阴性、螺旋形、能动细菌，命名为 NIO-S6(T) 菌株，从印度泰米尔纳德邦拉梅斯瓦拉姆近海的沉积物样本中分离得到。NIO-S6(T) 菌株被发现氧化酶、DNase 和赖氨酸脱羧酶活性呈阳性，而过氧化酶、明胶酶、脂肪酶、鸟氨酸脱羧酶、硝酸盐还原酶、七叶苷酶、淀粉酶和脲酶活性呈阴性。脂肪酸主要由 C10:0 3OH、C16:0、C16:1 和 C18:1 组成。NIO-S6(T) 株含有 Q-8 作为主要的呼吸醌。NIO-S6(T) 株的 DNA G+C 含量为 49.5±0.6mol%。基于 NIO-S6(T) 株的 16S rRNA 基因序列的系统发育分析表明，海洋螺旋菌属（Proteobacteria 门）的海洋螺旋菌和海洋螺旋菌是最接近的相关种，序列相似性分别为 98.4%和 97.8%。其他家族成员的序列相似性<96.4%。然而，与海洋螺旋菌 LMG 5214(T)和海洋螺旋菌 LMG 5213(T)的 DNA-DNA 杂交显示，NIO-S6(T)株与 NIO-S6(T)株的相关性分别为 31.5%和 46.9%。基于表型特征和系统发育推断，NIO-S6(T) 株被提议为海洋螺旋菌属的一个新种，命名为海洋螺旋菌 nioense sp. nov.，其模式菌株为 NIO-S6(T) (=MTCC 11154(T) = KCTC 32008(T))。
