Laboratory of Plant Biotechnologies, Institute of Experimental Botany of the CAS, v.v.i. , Rozvojova 263, 165 02 Prague 6 - Lysolaje, Czech Republic.
Department of Catalysis and Reaction Engineering, Institute of Chemical Process Fundamentals of the CAS, v.v.i. , Rozvojova 135/1, 165 02 Prague 6 - Lysolaje, Czech Republic.
Environ Sci Technol. 2017 Sep 19;51(18):10814-10824. doi: 10.1021/acs.est.7b02265. Epub 2017 Sep 7.
Engineered nanoparticles (ENPs) exhibit unique properties advantageous in a number of applications, but they also represent potential health and environmental risks. In this study, we investigated the phytotoxic mechanism of CuO ENPs using transcriptomic analysis and compared this response with the response to CuO bulk particles and ionic Cu. Ionic Cu at the concentration of 0.16 mg L changed transcription of 2692 genes (p value of <0.001, fold change of ≥2) after 7 days of exposure, whereas CuO ENPs and bulk particles (both in the concentration of 10 mg L) altered the expression of 922 and 482 genes in Arabidopsis thaliana roots, respectively. The similarity between transcription profiles of plants exposed to ENPs and ionic Cu indicated that the main factor in phytotoxicity was the release of Cu ions from CuO ENPs after 7 days of exposure. The effect of Cu ions was evident in all treatments, as indicated by the down-regulation of genes involved in metal homeostasis and transport and the up-regulation of oxidative stress response genes. ENPs were more soluble than bulk particles, resulting in the up-regulation of metallochaperone-like genes or the down-regulation of aquaporins and metal transmembrane transporters that was also characteristic for ionic Cu exposure.
工程纳米颗粒（ENPs）表现出独特的性质，在许多应用中具有优势，但它们也代表了潜在的健康和环境风险。在这项研究中，我们使用转录组分析研究了氧化铜 ENPs 的植物毒性机制，并将其与氧化铜体颗粒和离子铜的反应进行了比较。离子铜在浓度为 0.16 毫克/升的条件下，暴露 7 天后改变了 2692 个基因的转录（p 值<0.001，倍数变化≥2），而氧化铜 ENPs 和体颗粒（浓度均为 10 毫克/升）分别改变了拟南芥根系中 922 个和 482 个基因的表达。暴露于 ENPs 和离子铜的植物转录谱之间的相似性表明，在暴露 7 天后，主要的植物毒性因素是氧化铜 ENPs 中 Cu 离子的释放。Cu 离子的作用在所有处理中都很明显，这表明与金属稳态和运输有关的基因下调，以及与氧化应激反应基因的上调。ENPs 比体颗粒更易溶解，导致金属伴侣样基因的上调或水通道蛋白和金属跨膜转运体的下调，这也是离子铜暴露的特征。
