School of Biomedical Sciences, University of Western Australia, Nedlands, Western Australia, Australia.
Translational Cancer Pathology Laboratory, Pathwest Laboratory Medicine WA, QEII Medical Centre, Nedlands, Western Australia, Australia.
J Clin Microbiol. 2017 Nov;55(11):3175-3182. doi: 10.1128/JCM.01155-17. Epub 2017 Aug 23.
The standard paradigm for microbiological testing is dependent on the presentation of a patient to a clinician. Tests are then requested based on differential diagnoses using the patient's symptoms as a guide. The era of high-throughput genomic methods has the potential to replace this model for the first time with what could be referred to as "hypothesis-free testing." This approach utilizes one of a variety of methodologies to obtain a sequence from potentially any nucleic acid in a clinical sample, without prior knowledge of its content. We discuss the advantages of such an approach and the challenges in making this a reality.
微生物学检测的标准模式依赖于患者向临床医生就诊。然后，根据患者的症状进行鉴别诊断，请求进行测试。高通量基因组方法的时代有可能首次用所谓的“无假设测试”来取代这种模式。这种方法利用各种方法中的一种从临床样本中的任何潜在核酸中获取序列，而无需事先了解其内容。我们讨论了这种方法的优势以及使其成为现实所面临的挑战。
