表达T细胞受体γ/δ异二聚体的T细胞杂交瘤的产生。
Production of a T cell hybridoma that expresses the T cell receptor gamma/delta heterodimer.
作者信息
Yokoyama W M, Koning F, Stingl G, Bluestone J A, Coligan J E, Shevach E M
出版信息
J Exp Med. 1987 Jun 1;165(6):1725-30. doi: 10.1084/jem.165.6.1725.
Abstract
We have produced a T cell hybridoma line by fusion of an IL-2-dependent, long-term T cell receptor (TCR) gamma/delta+ Thy-1+, bone marrow-derived, dendritic epidermal cell line to the BW5147 tumor line. The resultant hybridoma was rapidly growing, lymphokine independent, and expressed T3 in association with the TCR gamma/delta heterodimer. Several subclones of the hybridoma line produced easily detectable levels of IL-2 after stimulation by anti-T3 or Con A. The availability of these cloned cell lines should greatly facilitate further functional, biochemical, and molecular studies of the TCR delta chain.
摘要
我们通过将依赖白细胞介素-2的、长期存在的T细胞受体（TCR）γ/δ⁺、Thy-1⁺、骨髓来源的树突状表皮细胞系与BW5147肿瘤系融合，制备了一个T细胞杂交瘤系。所得杂交瘤生长迅速，不依赖淋巴因子，并与TCRγ/δ异二聚体一起表达T3。杂交瘤系的几个亚克隆在抗T3或刀豆蛋白A刺激后产生易于检测水平的白细胞介素-2。这些克隆细胞系的可得性应极大地促进对TCRδ链的进一步功能、生化和分子研究。
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