Surachat Komwit, Sangket Unitsa, Deachamag Panchalika, Chotigeat Wilaiwan
Department of Molecular Biotechnology and Bioinformatics, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
Information and Communication Technology Programme, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
PLoS One. 2017 Aug 24;12(8):e0183548. doi: 10.1371/journal.pone.0183548. eCollection 2017.
Lactobacillus paracasei SD1 is a potential probiotic strain due to its ability to survive several conditions in human dental cavities. To ascertain its safety for human use, we therefore performed a comprehensive bioinformatics analysis and characterization of the bacterial protein toxins produced by this strain. We report the complete genome of Lactobacillus paracasei SD1 and its comparison to other Lactobacillus genomes. Additionally, we identify and analyze its protein toxins and antimicrobial proteins using reliable online database resources and establish its phylogenetic relationship with other bacterial genomes. Our investigation suggests that this strain is safe for human use and contains several bacteriocins that confer health benefits to the host. An in silico analysis of protein-protein interactions between the target bacteriocins and the microbial proteins gtfB and luxS of Streptococcus mutans was performed and is discussed here.
副干酪乳杆菌SD1因其能够在人类牙洞中多种条件下存活而成为一种潜在的益生菌菌株。为确定其对人类使用的安全性，我们因此对该菌株产生的细菌蛋白毒素进行了全面的生物信息学分析和表征。我们报告了副干酪乳杆菌SD1的完整基因组及其与其他乳杆菌基因组的比较。此外，我们使用可靠的在线数据库资源鉴定并分析了其蛋白毒素和抗菌蛋白，并建立了它与其他细菌基因组的系统发育关系。我们的研究表明，该菌株对人类使用是安全的，并且含有几种对宿主有益健康的细菌素。本文还进行并讨论了目标细菌素与变形链球菌的微生物蛋白gtfB和luxS之间蛋白质-蛋白质相互作用的计算机模拟分析。
