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鹰嘴豆中核苷酸结合位点富含亮氨酸重复序列基因家族的全基因组鉴定及组织特异性表达分析

Genome-wide identification and tissue-specific expression analysis of nucleotide binding site-leucine rich repeat gene family in (kabuli chickpea).

作者信息

Sharma Ranu, Rawat Vimal, Suresh C G

机构信息

Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, Maharashtra, India.

Institute of Plant and Microbial Biology and Zurich-Basel Plant Science Center, University of Zurich, Zollikerstrasse, 107, CH 8008, Zurich, Switzerland.

出版信息

Genom Data. 2017 Aug 12;14:24-31. doi: 10.1016/j.gdata.2017.08.004. eCollection 2017 Dec.

DOI:10.1016/j.gdata.2017.08.004
PMID:28840100
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5558467/
Abstract

The nucleotide binding site-leucine rich repeat (NBS-LRR) proteins play an important role in the defense mechanisms against pathogens. Using bioinformatics approach, we identified and annotated 104 NBS-LRR genes in chickpea. Phylogenetic analysis points to their diversification into two families namely TIR-NBS-LRR and non-TIR-NBS-LRR. Gene architecture revealed intron gain/loss events in this resistance gene family during their independent evolution into two families. Comparative genomics analysis elucidated its evolutionary relationship with other species. Around 50% NBS-LRRs reside in macro-syntenic blocks underlining positional conservation along with sequence conservation of NBS-LRR genes in chickpea. Transcriptome sequencing data provided evidence for their transcription and tissue-specific expression. Four -regulatory elements namely WBOX, DRE, CBF, and GCC boxes, that commonly occur in resistance genes, were present in the promoter regions of these genes. Further, the findings will provide a strong background to use candidate disease resistance NBS-encoding genes and identify their specific roles in chickpea.

摘要

核苷酸结合位点富含亮氨酸重复序列（NBS-LRR）蛋白在抵御病原体的防御机制中发挥着重要作用。利用生物信息学方法，我们在鹰嘴豆中鉴定并注释了104个NBS-LRR基因。系统发育分析表明它们可分为两个家族，即TIR-NBS-LRR和非TIR-NBS-LRR。基因结构揭示了该抗性基因家族在独立进化为两个家族的过程中发生的内含子增减事件。比较基因组学分析阐明了其与其他物种的进化关系。约50%的NBS-LRRs位于宏观共线性区域，这突出了鹰嘴豆中NBS-LRR基因在位置和序列上的保守性。转录组测序数据为它们的转录和组织特异性表达提供了证据。这些基因的启动子区域存在四种抗性基因中常见的调控元件，即WBOX、DRE、CBF和GCC框。此外，这些发现将为利用候选抗病NBS编码基因并确定它们在鹰嘴豆中的具体作用提供有力的背景支持。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcbc/5558467/319f6884b3f7/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcbc/5558467/f3338a911002/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcbc/5558467/468464eb0907/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcbc/5558467/8a08f3c68f5f/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcbc/5558467/319f6884b3f7/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcbc/5558467/f3338a911002/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcbc/5558467/468464eb0907/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcbc/5558467/8a08f3c68f5f/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcbc/5558467/319f6884b3f7/gr3.jpg

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