Chernikova Tatyana N, Dallimore James, Lünsdorf Heinrich, Heipieper Hermann J, Golyshin Peter N
School of Biological Sciences, Bangor University, Deiniol Road, Gwynedd LL57 2UW, UK.
Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany.
Int J Syst Evol Microbiol. 2017 Sep;67(9):3310-3317. doi: 10.1099/ijsem.0.002111. Epub 2017 Aug 25.
The novel Gram-negative, aerobic, non-motile, non-spore-forming, short-rod bacterium, strain C7T, was isolated from a seawater sample from Menai Straits (Wales, UK) and characterized. Phylogenetic analysis of 16S rRNA gene sequences showed that this strain represented a distinct lineage within the Roseobacterclade of family Rhodobacteracea within Alphaproteobacteria. The members of the genera Pontivivens (Pontivivensinsulae GYSW-23T), Celeribacter (Celeribactermanganoxidans DY2-5T), Donghicola (Donghicola eburneus SW-277T), Roseovarius (Roseovariushalotolerans HJ50T and Roseovariuspacificus 81-2T), Cribrihabitans (Cribrihabitansmarinus CZ-AM5T) and Aestuariihabitans (Aestuariihabitansbeolgyonensis BB-MW15T) were the closest relatives with 16S rRNA gene sequence identities between 93.4 and 95.6 %. Strain C7T could utilize a restricted number of complex substrates with a preference for yeast extract and tryptone, which is consistent with earlier observations that peptides may serve as an important energy and carbon source for bacteria from the Roseobacterclade. Growth occurred in the absence of sodium ions. The isolate C7T is a mesophilic bacterium that optimally grows at 20 °C. The strain can grow under microaerophilic conditions. The major fatty acid was C18 : 1cis d11. The only detected ubiquinone was Q10. The polar lipids of strain C7T were phosphatidylglycerol, two unknown aminolipids and three unknown lipids. The DNA G+C content of the strain was 60.0 mol%. Based on the results of the morphological, physiological and phylogenetic analyses, the new genus, Monaibacterium gen. nov., to include the new species Monaibacterium marinum sp. nov., is proposed. Strain C7T (=DSM 100241T, =LMG 28800T) is the type and only strain of M. marinum.
从英国威尔士梅奈海峡的海水样本中分离出一种新型革兰氏阴性、需氧、无运动性、不形成芽孢的短杆菌C7T，并对其进行了特性鉴定。16S rRNA基因序列的系统发育分析表明，该菌株代表了变形菌门红杆菌科玫瑰杆菌属内一个独特的谱系。Pontivivens属（Pontivivensinsulae GYSW-23T）、Celeribacter属（Celeribactermanganoxidans DY2-5T）、Donghicola属（Donghicola eburneus SW-277T）、Roseovarius属（Roseovariushalotolerans HJ50T和Roseovariuspacificus 81-2T）、Cribrihabitans属（Cribrihabitansmarinus CZ-AM5T）和Aestuariihabitans属（Aestuariihabitansbeolgyonensis BB-MW15T）的成员是其最亲近的亲缘关系菌，16S rRNA基因序列同一性在93.4%至95.6%之间。菌株C7T能够利用数量有限的复杂底物，偏好酵母提取物和胰蛋白胨，这与早期观察结果一致，即肽可能是玫瑰杆菌属细菌的重要能量和碳源。在没有钠离子的情况下也能生长。分离株C7T是嗜温菌，最适生长温度为20°C。该菌株可在微需氧条件下生长。主要脂肪酸为C18:1cis d11。唯一检测到的泛醌是Q10。菌株C7T的极性脂质为磷脂酰甘油、两种未知氨基脂质和三种未知脂质。该菌株的DNA G+C含量为60.0mol%。基于形态、生理和系统发育分析结果，提出了新属Monaibacterium gen. nov.，其中包括新种Monaibacterium marinum sp. nov.。菌株C7T（=DSM 100241T，=LMG 28800T）是M. marinum的模式菌株且是唯一菌株。
