Department of Microbiology, Pukyong National University, Busan, 48513, Republic of Korea.
School of Marine and Fisheries Life Science, Pukyong National University, Busan, 48513, Republic of Korea.
J Microbiol. 2021 Jun;59(6):546-551. doi: 10.1007/s12275-021-0678-7. Epub 2021 Apr 20.
A novel bacterium, designated strain RR4-56, was isolated from a biofilter of a seawater recirculating aquaculture system. The 16S rRNA gene sequence analysis showed that the isolate was closely related to Halovulum dunhuangense YYQ-30 (92.6%), Albimonas donghaensis DS2 (91.3%), Pontivivens insulae GYSW-23 (91.3%), and Monaibacterium marinum C7 (90.9%), belonging to the family Rhodobacteraceae. The strain was aerobic, Gram-negative, rod-shaped, oxidase-positive, and catalase-negative. Its optimum temperature, pH, and salinity for growth were 25-30°C, pH 8.5, and 2-3% NaCl (w/v), respectively. Its growth occurred at 15-35°C, pH 5.0-9.5, and 0-7% NaCl (w/v). It contained ubiquinone-10 (Q-10), a respiratory quinone, and the major cellular fatty acids were 11-methyl C ω7c (31.9%), C ω6c (30.4%), and C cyclo ω8c (16.1%). The polar lipids present in the strain were phosphatidylglycerol, an unidentified phospholipid, and an unidentified aminolipid. The strain had one 4,373,045 bp circular chromosome with G + C contents of 65.9 mol% including 4,169 genes, 4,118 coding sequences (CDSs), 3 rRNAs, and 45 tRNAs. Genome annotation predicted some gene clusters related to the degradation of several types of organic matter such as protocatechuate, catechol, and phthalate. Based on the polyphasic characteristics, RR4-56 represents a novel genus and species in the family Rhodobacteraceae, for which the name Pikeienuella piscinae gen. nov., sp. nov. was proposed. The type strain is RR4-56 (= KCTC 52648 = DSM 107918).
一种新型细菌，命名为 RR4-56 菌株，从海水再循环水产养殖系统的生物滤池中分离得到。16S rRNA 基因序列分析表明，该分离株与 Dunhuangense 属的 Halovulum dunhuangense YYQ-30（92.6%）、Donghaensis 属的 Albimonas donghaensis DS2（91.3%）、Insulae 属的 Pontivivens insulae GYSW-23（91.3%）和 Marinum 属的 Monaibacterium marinum C7（90.9%）密切相关，属于红杆菌科。该菌株为需氧、革兰氏阴性、杆状、氧化酶阳性、接触酶阴性。其最适生长温度、pH 值和盐度分别为 25-30°C、pH8.5 和 2-3%NaCl（w/v）。其生长温度为 15-35°C、pH5.0-9.5 和 0-7%NaCl（w/v）。它含有醌-10（Q-10），一种呼吸醌，主要细胞脂肪酸为 11-甲基 C ω7c（31.9%）、C ω6c（30.4%）和 C 环 ω8c（16.1%）。该菌株存在的极性脂为磷脂酰甘油、一种未鉴定的磷脂和一种未鉴定的胺脂。该菌株有一个 4,373,045 bp 的圆形染色体，G+C 含量为 65.9 mol%，包含 4,169 个基因、4,118 个编码序列（CDSs）、3 rRNA 和 45 tRNA。基因组注释预测了一些与几种类型的有机物降解有关的基因簇，如原儿茶酸、儿茶酚和邻苯二甲酸。基于多相特征，RR4-56 代表红杆菌科的一个新属和新种，建议命名为 Pikeienuella piscinae gen. nov.，sp. nov.。模式菌株为 RR4-56（=KCTC 52648=DSM 107918）。
