G.B. Elyakov Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch, Russian Academy of Sciences, Prospekt 100 Let Vladivostoku 159, Vladivostok, Russia, 690022.
Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeongeup-si, Jeollabuk-do, 56212, Republic of Korea.
Arch Microbiol. 2022 Jul 14;204(8):487. doi: 10.1007/s00203-022-03091-6.
A novel Gram-staining negative, strictly aerobic, rod-shaped, and non-motile bacterium, designated strain 9Alg 56, was isolated from the red alga Tichocarpus crinitus. The phylogenetic analysis based on 16S rRNA gene sequences placed the novel strain within the family Rhodobacteraceae, the order Rhodobacterales, the class Alphaproteobacteria, the phylum Pseudomonadota. The nearest neighbors of the new strain were Pontivivens insulae KCTC 42458, Oceanibium sediminis KCTC 62076, Halovulum dunhuangense YYQ-30 and Monaibacterium marinum C7 with 16S rRNA gene sequence similarity of 94.7, 94.4%, 93.1 and 92.7%, respectively. The AAI/ANI/dDDH values between 9Alg 56 and the five species of the closest genera (Pontivivens, Oceanibium, Halovulum, Monaibacterium, and 'Oceanomicrobium') were 58.63-63.91%/ 75.91-77.37%/ 19.3-20.4%. The prevalent fatty acids of strain 9Alg 56 were C ω7c, C and C 3-OH. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, phosphatidic acid, phosphatidylcholine, and two unidentified lipids. The DNA G+C content of strain 9Alg 56 was 61.5 mol%. A combination of the genotypic and phenotypic data showed that the algal isolate represents a novel genus and species, for which the name Algicella marina gen. nov., sp. nov. is proposed. The type strain is 9Alg 56 (= KCTC 72005 = KMM 6775).
一株新的革兰氏阴性、严格需氧、杆状、非运动细菌，被命名为Algicella marina 属的海洋菌，从红藻 Tichocarpus crinitus 中分离得到。基于 16S rRNA 基因序列的系统发育分析将新菌株置于红杆菌科、红杆菌目、α变形菌纲、假单胞菌门。新菌株的最近邻是 Pontivivens insulae KCTC 42458、Oceanibium sediminis KCTC 62076、Halovulum dunhuangense YYQ-30 和 Monaibacterium marinum C7，其 16S rRNA 基因序列相似性分别为 94.7%、94.4%、93.1%和 92.7%。9Alg 56 与五个最接近属（Pontivivens、Oceanibium、Halovulum、Monaibacterium 和 'Oceanomicrobium'）的 AAI/ANI/dDDH 值分别为 58.63-63.91%/75.91-77.37%/19.3-20.4%。菌株 9Alg 56 的优势脂肪酸为 C ω7c、C 和 C 3-OH。极性脂谱由磷脂酰乙醇胺、磷脂酰甘油、磷脂酸、磷脂酰胆碱和两种未鉴定的脂组成。菌株 9Alg 56 的 DNA G+C 含量为 61.5 mol%。基因型和表型数据的综合表明，该藻类分离株代表了一个新的属和种，建议将其命名为 Algicella marina 属，海洋菌种。模式菌株为 9Alg 56（=KCTC 72005=KMM 6775）。
