Park Sooyeon, Jung Yong-Taek, Park Ji-Min, Yoon Jung-Hoon
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Sep;67(9):3480-3484. doi: 10.1099/ijsem.0.002145. Epub 2017 Aug 25.
A Gram-stain-negative, non-motile, aerobic and ovoid or rod-shaped bacterium, designated JSSK-17T, was isolated from the place where the ocean and a freshwater spring meet at Jeju island, South Korea. Strain JSSK-17T grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 1.0-2.0 % (w/v) NaCl. Phylogenetic trees, based on 16S rRNA gene sequences, showed that strain JSSK-17T clustered with the type strains of Puniceibacterium antarcticum and Puniceibacterium sediminis, exhibiting sequence similarities of 98.1-98.2 %. Strain JSSK-17T and the type strains of P. antarcticum and P. sediminis contained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the major fatty acid. The predominant polar lipids detected in strain JSSK-17T and the type strains of P. antarcticum and P. sediminis were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified aminolipid, one unidentified glycolipid and one unidentified phospholipid. The DNA G+C content of strain JSSK-17T was 64.4 mol% and its DNA-DNA relatedness values with the type strains of P. antarcticum and P. sediminis were 24 and 15 %, respectively. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain JSSK-17T is separated from the two reference species of the genus Puniceibacteriumwith validly published names. On the basis of the data presented, strain JSSK-17T is considered to represent a novel species of the genus Puniceibacterium, for which the name Puniceibacterium confluentis sp. nov. is proposed. The type strain is JSSK-17T (=KCTC 52765T=NBRC 112766T).
从韩国济州岛海洋与淡水泉交汇处分离出一株革兰氏染色阴性、无运动性、需氧的卵形或杆状细菌，命名为JSSK-17T。菌株JSSK-17T在30°C、pH 7.0 - 8.0以及1.0 - 2.0%（w/v）NaCl条件下生长最佳。基于16S rRNA基因序列构建的系统发育树表明，菌株JSSK-17T与南极海红杆菌（Puniceibacterium antarcticum）和沉积物海红杆菌（Puniceibacterium sediminis）的模式菌株聚类在一起，序列相似性为98.1 - 98.2%。菌株JSSK-17T以及南极海红杆菌和沉积物海红杆菌的模式菌株以Q-10作为主要泛醌，以C18:1ω7c作为主要脂肪酸。在菌株JSSK-17T以及南极海红杆菌和沉积物海红杆菌的模式菌株中检测到的主要极性脂类为磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油、二磷脂酰甘油、一种未鉴定的氨基脂类、一种未鉴定的糖脂类和一种未鉴定的磷脂类。菌株JSSK-17T的DNA G+C含量为64.4 mol%，其与南极海红杆菌和沉积物海红杆菌模式菌株的DNA-DNA相关性值分别为24%和15%。这些差异表型特征，连同系统发育和遗传独特性，表明菌株JSSK-17T与已有效发表名称的海红杆菌属的两个参考物种不同。根据所提供的数据，菌株JSSK-17T被认为代表海红杆菌属的一个新物种，为此提出名为融合海红杆菌（Puniceibacterium confluentis）的新种。模式菌株为JSSK-17T（=KCTC 52765T=NBRC 112766T）。
