Cline H T, Debski E A, Constantine-Paton M
Proc Natl Acad Sci U S A. 1987 Jun;84(12):4342-5. doi: 10.1073/pnas.84.12.4342.
The optic tecta of surgically produced three-eyed tadpoles were chronically exposed to the N-methyl-D-aspartate (NMDA) receptor antagonist aminophosphonovaleric acid (APV), or to NMDA itself, to assess the influence of NMDA receptor/channels on the eye-specific segregation of retinal ganglion cell (RGC) terminals that occurs whenever two retinas innervate one tectal lobe. Exposure of the tectum to the active isomer of APV produces desegregation of the RGC terminals without blocking electrical activity in the afferents or altering their terminal arbor morphology. Exposure to the inactive isomer of APV causes no perturbation of the normal stripe pattern. APV-induced desegregation is completely reversible within 2 weeks of removal of the APV. In addition, exposure of the optic tectum to NMDA results in stripes with sharper borders and fewer forks and fusions than untreated animals. These results suggest that the NMDA receptor/channel plays a role in eye-specific segregation in the three-eyed tadpole.
将通过手术制造出的三只眼蝌蚪的视顶盖长期暴露于N-甲基-D-天冬氨酸（NMDA）受体拮抗剂氨磷戊酸（APV）或NMDA自身，以评估NMDA受体/通道对视网膜神经节细胞（RGC）终末的眼特异性分离的影响，这种分离在两个视网膜支配一个视叶时都会发生。将视顶盖暴露于APV的活性异构体可使RGC终末去分离，而不会阻断传入神经中的电活动或改变其终末分支形态。暴露于APV的非活性异构体不会干扰正常的条纹模式。在去除APV后的2周内，APV诱导的去分离是完全可逆的。此外，与未处理的动物相比，将视顶盖暴露于NMDA会导致条纹边界更清晰，分叉和融合更少。这些结果表明，NMDA受体/通道在三眼蝌蚪的眼特异性分离中起作用。
