Christian M S, Hoberman A M, Lochry E A
Teratog Carcinog Mutagen. 1987;7(1):65-71. doi: 10.1002/tcm.1770070109.
The Chernoff-Kavlock assay was initially designed to identify developmental toxins within a relatively short time frame, using a mammalian system. Although it has been used for initial prioritization of multiple agents studied concurrently or for dosage-range evaluations, it has not gained wide usage in a commercial setting. As proposed, use of the Chernoff-Kavlock assay in an industrial setting is relatively inefficient, in terms of animal usage and data produced, when compared with available alternative study designs. Only a single dosage level was studied, and the data obtained from the assay do not provide sufficient information to meet the minimum requirements of safety evaluations submitted for regulatory review. For these reasons, other methods of prioritization are generally used. Alternate methods used for initial screening and prioritization at our laboratory are: 1) the hydra developmental toxicity assay; and 2) a dosage-range study in rats. When the results of several pilot and definitive developmental toxicity assays performed in rats were compared, it appeared that the described pilot study served the dual functions of predicting and prioritizing the developmental hazard of the test agent and providing a dosage-range study that identified the appropriate dosages to be tested in the definitive study.
切尔诺夫-卡夫洛克试验最初旨在利用哺乳动物系统在相对较短的时间内识别发育毒素。尽管它已被用于对同时研究的多种药剂进行初步优先级排序或剂量范围评估，但在商业环境中尚未得到广泛应用。如所提议的，与现有的替代研究设计相比，在工业环境中使用切尔诺夫-卡夫洛克试验在动物使用和产生的数据方面效率相对较低。仅研究了单一剂量水平，并且从该试验获得的数据不足以提供满足提交给监管审查的安全性评估最低要求的信息。由于这些原因，通常使用其他优先级排序方法。在我们实验室用于初步筛选和优先级排序的替代方法是：1）水螅发育毒性试验；2）大鼠剂量范围研究。当比较在大鼠中进行的几项预试验和确定性发育毒性试验的结果时，似乎所描述的预试验起到了预测受试物发育危害并对其进行优先级排序以及提供剂量范围研究以确定在确定性研究中要测试的合适剂量的双重作用。
