Wickramaratne G A
Teratog Carcinog Mutagen. 1987;7(1):73-83. doi: 10.1002/tcm.1770070110.
As the rat is the preferred species for teratogenicity studies in our laboratories, the Chernoff-Kavlock assay was modified and validated for use in this species. The Alpk:AP (Wistar-derived) strain, which is also used routinely for toxicology studies, was used, under standard SPF conditions. Twenty-six chemicals have been assayed to demonstrate the utility of the system as a predictor of rat teratogens and to develop a set of rules for interpretation of the results. Routes of chemical administration were largely gavage, with some dermal, subcutaneous, inhalation, and intraperitoneal applications. The use of the assay in five modes to assist the toxicological evaluation or understanding of chemicals is displayed. These modes are 1) as a pre-screen, 2) to compare analogues/homologues, 3) to provide a rapid hazard estimate, 4) to generate a dose-response, and 5) to test a hypothesis.
由于大鼠是我们实验室致畸性研究的首选物种，因此对切尔诺夫-卡夫洛克试验进行了修改并验证可用于该物种。在标准无特定病原体条件下，使用了也常用于毒理学研究的Alpk:AP（源自Wistar）品系。已对26种化学物质进行了检测，以证明该系统作为大鼠致畸物预测指标的效用，并制定一套结果解释规则。化学物质的给药途径主要是灌胃，也有一些经皮、皮下、吸入和腹腔内给药。展示了该试验在五种模式下用于协助化学物质毒理学评估或理解的情况。这些模式分别是：1）作为预筛选，2）比较类似物/同系物，3）提供快速危害估计，4）生成剂量反应，5）检验假设。
