Effects of reduced energy availability on bone metabolism in women and men.

BACKGROUND

The short-term effects of low energy availability (EA) on bone metabolism in physically active women and men are currently unknown.

PURPOSE

We evaluated the effects of low EA on bone turnover markers (BTMs) in a cohort of women and a cohort of men, and compared effects between sexes.

METHODS

These studies were performed using a randomised, counterbalanced, crossover design. Eleven eumenorrheic women and eleven men completed two 5-day protocols of controlled (CON; 45kcal·kgLBM·d) and restricted (RES; 15kcal·kgLBM·d) EAs. Participants ran daily on a treadmill at 70% of their peak aerobic capacity (VO peak) resulting in an exercise energy expenditure of 15kcal·kgLBM·d and consumed diets providing 60 and 30kcal·kgLBM·d. Blood was analysed for BTMs [β-carboxyl-terminal cross-linked telopeptide of type I collagen (β-CTX) and amino-terminal propeptide of type 1 procollagen (P1NP)], markers of calcium metabolism [parathyroid hormone (PTH), albumin-adjusted calcium (ACa), magnesium (Mg) and phosphate (PO)] and regulatory hormones [sclerostin, insulin-like growth factor 1 (IGF-1), triiodothyronine (T), insulin, leptin, glucagon-like-peptide-2 (GLP-2)].

RESULTS

In women, β-CTX AUC was significantly higher (P=0.03) and P1NP AUC was significantly lower (P=0.01) in RES compared to CON. In men, neither β-CTX (P=0.46) nor P1NP (P=0.12) AUCs were significantly different between CON and RES. There were no significant differences between sexes for any BTM AUCs (all P values>0.05). Insulin and leptin AUCs were significantly lower following RES in women only (for both P=0.01). There were no differences in any AUCs of regulatory hormones or markers of calcium metabolism between men and women following RES (all P values>0.05).

CONCLUSIONS

When comparing within groups, five days of low EA (15kcal·kgLBM·d) decreased bone formation and increased bone resorption in women, but not in men, and no sex specific differences were detected.