Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
Faculty of Veterinary Medicine, Mahasarakham University, Mahasarakham, 44000, Thailand.
Adv Exp Med Biol. 2017;975 Pt 2:769-787. doi: 10.1007/978-94-024-1079-2_60.
This study tests the hypothesis that perinatal taurine imbalance impairs renal function in adult female rats via alterations in estrogen activity. Female Sprague-Dawley rats were fed normal rat chow and water containing 3% beta-alanine (TD), 3% taurine (TS) or water alone (C) from conception until weaning. Then, female offspring received normal rat chow and water with (CG, TDG, TSG) or without (CW, TDW, TSW) 5% glucose. At 7-8 weeks of age, renal function at rest and after acute saline load was tested in conscious, restrained female rats treated with non-selective estrogen receptor blocker tamoxifen for a week. Compared to control, TD or TS did not affect mean arterial pressure (MAP). Tamoxifen significantly increased resting MAP only in TDG compared to TDW groups. Although renal blood flow did not significantly differ among the groups, renal vascular resistance increased in TSG compared to CW, CG, and TSW groups. Glomerular filtration rate and water and sodium excretion were not significantly different among the groups. Compared to CW, saline load significantly depressed fractional water excretion in CG, TDW, TDG, and TSW, and fractional sodium excretion in CG, TDW, TDG, TSW, and the TSG groups. Potassium excretion was not significantly different among the corresponding groups. Fractional potassium excretion significantly increased in TDW compared to CG and in TSG compared to CG and TSW groups. These differences were abolished by tamoxifen treatment. These data indicate that in adult female rats, perinatal taurine imbalance, particularly followed by high sugar intake, alters renal function via an estrogenic mechanism.
这项研究检验了这样一个假设，即围产期牛磺酸失衡通过改变雌激素活性损害成年雌性大鼠的肾功能。雌性 Sprague-Dawley 大鼠从受孕到断奶期间，喂食正常大鼠饲料和含有 3%β-丙氨酸（TD）、3%牛磺酸（TS）或单独水（C）的水。然后，雌性后代接受正常大鼠饲料和水，其中含有（CG、TDG、TSG）或不含有（CW、TDW、TSW）5%葡萄糖。在 7-8 周龄时，在接受非选择性雌激素受体阻滞剂他莫昔芬治疗一周的清醒、束缚的雌性大鼠中，测试静息和急性盐水负荷后的肾功能。与对照组相比，TD 或 TS 并不影响平均动脉压（MAP）。与 TDW 组相比，他莫昔芬仅在 TDG 组中显著增加静息 MAP。尽管各组之间的肾血流量没有显著差异，但与 CW、CG 和 TSW 组相比，TSG 组的肾血管阻力增加。肾小球滤过率和水钠排泄在各组之间没有显著差异。与 CW 相比，盐水负荷显著抑制 CG、TDW、TDG 和 TSW 组的分数水排泄，以及 CG、TDW、TDG、TSW 和 TSG 组的分数钠排泄。相应组之间的钾排泄没有显著差异。与 CG 相比，TDW 组的分数钾排泄显著增加，与 CG 和 TSW 相比，TSG 组的分数钾排泄显著增加。这些差异通过他莫昔芬治疗被消除。这些数据表明，在成年雌性大鼠中，围产期牛磺酸失衡，特别是随后高糖摄入，通过雌激素机制改变肾功能。
