Department of Physiology, Khon Kaen University, Khon Kaen, Thailand.
Adv Exp Med Biol. 2013;776:67-80. doi: 10.1007/978-1-4614-6093-0_8.
Perinatal taurine depletion followed by high sugar intake (postweaning) alters the renin-angiotensin system (RAS) and glucose regulation in adult female rats. This study tests the hypothesis that in adult female rats, RAS and estrogen contribute to insulin resistance resulting from perinatal taurine imbalance. Female Sprague-Dawley rats were fed normal rat chow with 3% β-alanine (taurine depletion, TD), 3% taurine (taurine supplementation, TS), or water alone (control, C) from conception to weaning. Their female offspring were fed normal rat chow with 5% glucose in water (TDG, TSG, CG) or water alone (TDW, TSW, CW) throughout the experiment. At 7-8 weeks of age, animals were studied with or without captopril inhibition of the RAS and with or without estrogen receptor inhibition by tamoxifen. Compared to CW and CG groups, perinatal taurine depletion but not supplementation slightly increased plasma insulin levels. High sugar intake slightly increased plasma insulin only in TSG. Captopril treatment significantly increased plasma insulin in all groups except CG (the greatest increase was in TDG). Changes in insulin resistance and insulin secretion paralleled the changes in plasma insulin levels. In contrast, tamoxifen treatment increased insulin resistance and decreased insulin secretion only in TDG and this group displayed hyperglycemia and glucose intolerance. These data indicate that perinatal taurine imbalance alters the interplay of RAS and estrogen on glucose-insulin regulation in adult female rats.
围产期牛磺酸耗竭随后高糖摄入（断奶后）改变成年雌性大鼠的肾素-血管紧张素系统（RAS）和葡萄糖调节。本研究检验了以下假设：在成年雌性大鼠中，RAS 和雌激素有助于由围产期牛磺酸失衡引起的胰岛素抵抗。雌性 Sprague-Dawley 大鼠从受孕到断奶期间喂食含 3%β-丙氨酸（牛磺酸耗竭，TD）、3%牛磺酸（牛磺酸补充，TS）或单独水（对照，C）的正常大鼠饲料。它们的雌性后代在整个实验过程中均喂食含 5%葡萄糖的正常大鼠饲料或单独水（TDG、TSG、CG）或单独水（TDW、TSW、CW）。在 7-8 周龄时，动物在有或无 RAS 抑制物卡托普利和有或无雌激素受体抑制剂他莫昔芬的情况下进行研究。与 CW 和 CG 组相比，围产期牛磺酸耗竭但不是补充稍微增加了血浆胰岛素水平。高糖摄入仅在 TSG 中略微增加了血浆胰岛素。卡托普利处理在除 CG 组（最大增加在 TDG 组）外的所有组中均显著增加了血浆胰岛素。胰岛素抵抗和胰岛素分泌的变化与血浆胰岛素水平的变化平行。相比之下，他莫昔芬处理仅在 TDG 组中增加了胰岛素抵抗并降低了胰岛素分泌，该组表现出高血糖和葡萄糖耐量降低。这些数据表明，围产期牛磺酸失衡改变了 RAS 和雌激素对成年雌性大鼠葡萄糖-胰岛素调节的相互作用。
