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淋巴管内皮细胞对 VEGFR-3 和 VEGFR-2 刺激的独特转录反应。

Distinct transcriptional responses of lymphatic endothelial cells to VEGFR-3 and VEGFR-2 stimulation.

机构信息

Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology (ETH) Zurich, 8093 Zurich, Switzerland.

RIKEN Center for Life Science Technologies, Division of Genomic Technologies, Yokohama, Kanagawa 230-0045, Japan.

出版信息

Sci Data. 2017 Aug 29;4:170106. doi: 10.1038/sdata.2017.106.

DOI:10.1038/sdata.2017.106
PMID:28850122
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5574372/
Abstract

Vascular endothelial growth factors (VEGFs) and their receptors play crucial roles in the formation of blood and lymphatic vessels during embryogenesis, and also under pathologic conditions in the adult. Despite intensive efforts over the last decades to elucidate the precise functions of VEGFs, transcriptional responses to VEGF receptor stimulation are still not fully characterized. To investigate the specific transcriptional effects of VEGFR-2 and VEGFR-3 activation, we performed a correlation analysis of previously published CAGE sequencing and microarray data of human lymphatic endothelial cells (LECs) stimulated with distinct VEGFs acting through either VEGFR-2 or VEGFR-3. We identified that specific activation of VEGFR-3 by VEGF-C156S results in the downregulation of many genes involved in immune regulation and inflammation, suggesting that VEGFR-3 stimulation has direct anti-inflammatory effects. Comparing CAGE and microarray data sets, we furthermore identified a small number of genes that showed a receptor-dependent response in LECs, demonstrating that these receptors, despite activating very similar signaling pathways, fulfill overlapping but not identical functions within the same cell type (LECs).

摘要

血管内皮生长因子（VEGFs）及其受体在胚胎发生过程中血液和淋巴管的形成中起着至关重要的作用，在成人的病理条件下也是如此。尽管在过去几十年中，人们为阐明 VEGFs 的精确功能做出了巨大努力，但 VEGF 受体刺激的转录反应仍未完全描述。为了研究 VEGFR-2 和 VEGFR-3 激活的特定转录效应，我们对先前发表的人类淋巴管内皮细胞（LEC）受通过 VEGFR-2 或 VEGFR-3 作用的不同 VEGFs 刺激的 CAGE 测序和微阵列数据进行了相关性分析。我们发现，VEGF-C156S 对 VEGFR-3 的特异性激活导致许多参与免疫调节和炎症的基因下调，表明 VEGFR-3 刺激具有直接的抗炎作用。比较 CAGE 和微阵列数据集，我们还鉴定出少数在 LEC 中表现出受体依赖性反应的基因，表明这些受体尽管激活非常相似的信号通路，但在同一细胞类型（LEC）中具有重叠但不完全相同的功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2561/5574372/eebd2512daf0/sdata2017106-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2561/5574372/92f223ba4c29/sdata2017106-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2561/5574372/368f279003a3/sdata2017106-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2561/5574372/d74b2acda59d/sdata2017106-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2561/5574372/eebd2512daf0/sdata2017106-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2561/5574372/92f223ba4c29/sdata2017106-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2561/5574372/368f279003a3/sdata2017106-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2561/5574372/d74b2acda59d/sdata2017106-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2561/5574372/eebd2512daf0/sdata2017106-f4.jpg

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3
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4
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7
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